Human Annexin V ELISA Kit (Colorimetric) Summary
Description |
The human Annexin V ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Annexin V. |
Specificity |
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human Annexin V positive serum. There was no crossreactivity detected, notably not with Annexin VIII (Vac beta). |
Standard Curve Range |
0.78 - 50 ng/mL |
Sensitivity |
0.33 ng/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 11.00% |
Intra-Assay |
CV% < 10% |
Spike Recovery |
The overall mean recovery was 85%. Please see protocol for more specific Spike Recovery details. |
Sample Volume |
50 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
ANXA5 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Annexin V
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Biotin-Conjugate anti-human Annexin V monoclonal antibody
- Human Annexin V Standard lyophilized, 100 ng/ml upon reconstitution
- Sample Diluent
- Stop Solution (1M Phosphoric acid)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
|
Alternate Names for Human Annexin V ELISA Kit (Colorimetric)
Background
Annexin V will bind to the phosphatidylserine-exposing apoptotic cell and can inhibit the procoagulant and proinflammatory activities of the dying cell. Total endogenous annexin V can be detected with the present ELISA which will offer further insights in the pathophysiological role of Annexin V.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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