Human Annexin V ELISA Kit (Colorimetric)

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ELISA: Annexin V ELISA Kit [NBP1-91253] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.33 ng/mL

Order Details

Human Annexin V ELISA Kit (Colorimetric) Summary

Specificity
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human Annexin V positive serum. There was no crossreactivity detected, notably not with Annexin VIII (Vac beta).
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.33 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
11.00%
Intra-Assay
10%
Spike Recovery
The overall mean recovery was 85%. Please see protocol for more specific Spike Recovery details.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ANXA5

Applications/Dilutions

Application Notes
The human Annexin V ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Annexin V.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Streptavidin-HRP
  2. Substrate Solution (tetramethyl-benzidine)
  3. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  4. Stop Solution (1M Phosphoric acid)
  5. Sample Diluent
  6. Human Annexin V Standard lyophilized, 100 ng/ml upon reconstitution
  7. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Annexin V
  8. Adhesive Films
  9. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  10. Biotin-Conjugate anti-human Annexin V monoclonal antibody

Alternate Names for Human Annexin V ELISA Kit (Colorimetric)

  • Anchorin CII
  • annexin A5
  • Annexin V
  • annexin-5
  • ANX5
  • ANXA5
  • ANXV
  • Calphobindin I
  • Endonexin II
  • ENX2CBP-I
  • Lipocortin V
  • PAP-I
  • Placental anticoagulant protein 4
  • Placental anticoagulant protein I
  • PP4
  • Thromboplastin inhibitor
  • VAC-alpha
  • Vascular anticoagulant-alpha

Background

Annexin V will bind to the phosphatidylserine-exposing apoptotic cell and can inhibit the procoagulant and proinflammatory activities of the dying cell. Total endogenous annexin V can be detected with the present ELISA which will offer further insights in the pathophysiological role of Annexin V.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ANXA5
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