Human Annexin V ELISA Kit (Colorimetric)

ELISA: Annexin V ELISA Kit [NBP1-91253] - Standard curve

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.33 ng/mL

Human Annexin V ELISA Kit (Colorimetric) Summary

• Description: The human Annexin V ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Annexin V.
• Specificity: The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human Annexin V positive serum. There was no crossreactivity detected, notably not with Annexin VIII (Vac beta).
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.33 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 11.00%
• Intra-Assay: CV% < 10%
• Spike Recovery: The overall mean recovery was 85%. Please see protocol for more specific Spike Recovery details.
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ANXA5

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Annexin V ELISA Kit (Colorimetric)

• Anchorin CII
• annexin A5
• Annexin V
• annexin-5
• ANX5
• ANXA5
• ANXV
• Calphobindin I
• Endonexin II
• ENX2CBP-I
• Lipocortin V
• PAP-I
• Placental anticoagulant protein 4
• Placental anticoagulant protein I
• PP4
• Thromboplastin inhibitor
• VAC-alpha
• Vascular anticoagulant-alpha

Background

Annexin V will bind to the phosphatidylserine-exposing apoptotic cell and can inhibit the procoagulant and proinflammatory activities of the dying cell. Total endogenous annexin V can be detected with the present ELISA which will offer further insights in the pathophysiological role of Annexin V.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Annexin V ELISA Kit (NBP1-91253). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ANXA5