Annexin A4 Antibody (JG37-83)

Western Blot: Annexin A4 Antibody (JG37-83) [NBP2-76976] - Western blot analysis of Annexin IV on human liver tissue lysate using anti-Annexin IV antibody at 1/2,000 dilution.

Immunocytochemistry/Immunofluorescence: Annexin A4 Antibody (JG37-83) [NBP2-76976] - ICC staining Annexin IV in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Annexin A4 Antibody (JG37-83) [NBP2-76976] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Annexin IV antibody. Counter stained with hematoxylin.

Flow Cytometry: Annexin A4 Antibody (JG37-83) [NBP2-76976] - Flow cytometric analysis of HepG2 cells with Annexin IV antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Annexin A4 Antibody (JG37-83) [NBP2-76976] - ICC staining Annexin IV in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Annexin A4 Antibody (JG37-83) [NBP2-76976] - ICC staining Annexin IV in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Annexin A4 Antibody (JG37-83) [NBP2-76976] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Annexin IV antibody. Counter stained with hematoxylin.

Product Discontinued

Annexin A4 Antibody (JG37-83) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Annexin A4 aa 10-107 / 319. (SwissProt: P09525 Human)
• Localization: Secreted. Nucleus. Plasma Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ANXA4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Annexin A4 Antibody (JG37-83)

• AIV
• Annexin A4
• annexin IV (placental anticoagulant protein II)
• Annexin IV
• annexin-4
• ANX4
• ANX4Carbohydrate-binding protein p33/p41
• ANXA4
• Chromobindin-4
• chromobindin-4,35-beta calcimedin
• DKFZp686H02120
• Endonexin I
• Lipocortin IV
• MGC75105
• P32.5
• PAP-II
• PIG28
• Placental anticoagulant protein II
• PP4-X
• proliferation-inducing gene 28
• proliferation-inducing protein 28
• Protein II
• ZAP36

Background

Annexin A4, also known as Annexin A4, belongs to the annexin family of calcium dependent phospholipid binding proteins. Annexin A4 is almost exclusively expressed in epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANXA4