Annexin A2 Antibody (ANEX 3D5/4) - BSA Free

• Reactivity: Hu
• Applications: WB, ELISA, IA, ICC/IF, IHC, IP
• Clone: ANEX 3D5/4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Annexin A2 Antibody (ANEX 3D5/4) - BSA Free Summary

• Immunogen: Mixture of native membrane proteins from human bone tissues which would include whole native annexin proteins
• Localization: Cell Membrane
• Specificity: ANEX 3D5/4 detects native protein. Recommended concentration range: 1-10 ug/ml.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANXA2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunoassay
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: Use in Immunoassay reported in scientific literature (PMID: 31035430).
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Annexin A2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Notes

Positive control(s): Endothelial cells.

Alternate Names for Annexin A2 Antibody (ANEX 3D5/4) - BSA Free

• Annexin A2
• Annexin II
• annexin-2
• ANX2
• ANX2L4
• ANX2L4LPC2
• ANXA2
• CAL1H
• Calpactin I heavy chain
• calpactin I heavy polypeptide
• Calpactin-1 heavy chain
• chromobindin 8
• chromobindin-8
• LIP2
• LIP2PAP-IV
• Lipocortin II
• Lipocortin-2
• LPC2D
• LPC2DP36
• p36
• PAP-IV
• Placental anticoagulant protein IV
• Protein I

Background

The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin II is a calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Annexin A2 Antibody (NB100-2724)(3)

Publications using NB100-2724

• Guo J, Yang WL, Pak D, et al. Osteopontin, Macrophage Migration Inhibitory Factor and Anti-Interleukin-8 Autoantibodies Complement CA125 for Detection of Early Stage Ovarian Cancer Cancers (Basel) 2019-04-28 [PMID: 31035430] (IA, Human)
• Zhang Y, Bi J, Zhu H et al. ANXA2 could act as a moderator of EGFR-directed therapy resistance in triple negative breast cancer Biosci. Biotechnol. Biochem. 2018-06-18 [PMID: 29912633] (IP, ICC/IF, Human)
• Liu C, Qu L, Lian S et al. Unconventional secretion of synuclein-gamma promotes tumor cell invasion. FEBS J. 2014-09-17 [PMID: 25229320]

Bioinformatics

• Gene Symbol: ANXA2
• Entrez:
  • 302(Human)