Annexin A11 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Annexin A11 Antibody [NB100-78588] - Colocalization of Sorcin with PDCD6 and annexins 7 and 11. Experiments showing co-localization between Sorcin (mouse alpha-sorcin, green) and annexin11 (top panel: rabbit alpha-annexin11, red), or annexin7 (bottom panel: rabbit alpha-annexin7, red), in 3T3-L1 preadipocytes in cytokinesis. Bars: 10 um. Note the colocalization in the midbody (arrows and insets). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep16828) licensed under a CC-BY license.

Western Blot: Annexin A11 Antibody [NB100-78588] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE; antibody diluted at 1:1000.

• Reactivity: Hu, Rb
• Applications: WB, ICC/IF, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Annexin A11 Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Annexin A11. The exact sequence is proprietary.
• Localization: Cytoplasmic
• Predicted Species: Rabbit (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANXA11
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Application Notes: ICC/IF usage reported in scientific literature (PMID: 26577048).
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Annexin A11 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26577048).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Protein A purified

Alternate Names for Annexin A11 Antibody - BSA Free

• Annexin A11
• Annexin-11
• ANX1156-kD
• ANXA11
• CAP50
• CAP-50

Background

ANXA11 (Annexin XI) was a member of the Ca2+-dependent, phospholipid-binding protein family, which showed a cell cycle-specific distribution from the cytoplasm to the mitotic spindle. In metaphase, annexin XI was up-regulated and costained with alpha-tubulin. ANXA11 was also shown a Ca2+ dependent distrubution in the nuclear and cytoplasmic domain. Annexin XI also shown 1) the regulation function of secretion or degranulation of neutrophil granules; 2) insulin release; 3)vesicle formation function in the cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Annexin A11 Antibody (NB100-78588)(2)

Publications using NB100-78588

• Ilari A, Fiorillo A, Poser E et al. Structural basis of Sorcin-mediated calcium-dependent signal transduction. Sci Rep 2015-01-01 [PMID: 26577048] (ICC/IF, Mouse)
• Lalioti VS, Ilari A, O'Connell DJ et al. Sorcin links calcium signaling to vesicle trafficking, regulates polo-like kinase 1 and is necessary for mitosis. PLoS ONE 2014-01-15 [PMID: 24427308]

Bioinformatics

• Gene Symbol: ANXA11
• Entrez:
  • 311(Human)