Annexin A11 Overexpression Lysate (Native)

Images

 
Western Blot: Annexin A11 Overexpression Lysate (Adult Normal) [NBL1-07559] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Annexin A11.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Annexin A11 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC223654 and based on accession number NM_145869. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens annexin A11 (ANXA11), transcript variant c, mRNA.
Gene
ANXA11

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
54.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Annexin A11

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Annexin A11 Overexpression Lysate (Native)

  • Annexin A11
  • Annexin-11
  • ANX1156-kD
  • ANXA11
  • CAP50
  • CAP-50

Background

This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain the calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Transcript variants encoding the same isoform have been identified. [provided by RefSeq]. Transcript Variant: This variant (c) differs in the 5' UTR compared to variant a. Variants a, b, and c encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-89388
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF3926
Species: Hu, Mu, Rt
Applications: WB
NB100-65678
Species: Hu, Mu, Rt, Xp
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP2-19741
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-52414
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready, ICC, IF
AF8105
Species: Hu
Applications: WB, IHC
NBP1-31310
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
AF7117
Species: Hu, Mu
Applications: WB, IHC, ICC
AF4149
Species: Hu, Mu
Applications: WB
H00011155-M06
Species: Hu
Applications: WB, ELISA, ICC/IF
NB100-304
Species: Hu, Mu, Rt, Fi, Gt, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
NBP2-13304
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-32665
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB600-1293
Species: Hu, Mu, Rt, Am, Bv, Ca, Ch, Tr
Applications: WB, ICC/IF, IHC, IHC-Fr
AF4146
Species: Hu, Mu, Rt
Applications: WB, IHC
NBP2-45224
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-27972
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBL1-07559
Species: Hu
Applications: WB

Publications for Annexin A11 Lysate (NBL1-07559) (0)

There are no publications for Annexin A11 Lysate (NBL1-07559).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Annexin A11 Lysate (NBL1-07559) (0)

There are no reviews for Annexin A11 Lysate (NBL1-07559). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Annexin A11 Lysate (NBL1-07559) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Annexin A11 Products

Bioinformatics Tool for Annexin A11 Lysate (NBL1-07559)

Discover related pathways, diseases and genes to Annexin A11 Lysate (NBL1-07559). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Annexin A11 Lysate (NBL1-07559)

Discover more about diseases related to Annexin A11 Lysate (NBL1-07559).
 

Pathways for Annexin A11 Lysate (NBL1-07559)

View related products by pathway.

Research Areas for Annexin A11 Lysate (NBL1-07559)

Find related products by research area.

Blogs on Annexin A11

There are no specific blogs for Annexin A11, but you can read our latest blog posts.

Customers Who Bought This Also Bought

53BP1 Antibody
NB100-304

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Annexin A11 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol ANXA11
Entrez