Annexin A11 Overexpression Lysate (Native)

Western Blot: Annexin A11 Overexpression Lysate (Adult Normal) [NBL1-07559] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Annexin A11.

• Reactivity: Hu
• Applications: WB

Annexin A11 Overexpression Lysate (Native) Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC223654 and based on accession number NM_145869. The protein contains a C-terminal DDK tag.
• Epitope: C-terminal Myc/DDK
• Specificity: Homo sapiens annexin A11 (ANXA11), transcript variant c, mRNA.
• Gene: ANXA11

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
• Theoretical MW: 54.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Annexin A11

• Type: Overexpression
• Protein State: Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Annexin A11 Overexpression Lysate (Native)

• Annexin A11
• Annexin-11
• ANX1156-kD
• ANXA11
• CAP50
• CAP-50

Background

This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain the calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Transcript variants encoding the same isoform have been identified. [provided by RefSeq]. Transcript Variant: This variant (c) differs in the 5' UTR compared to variant a. Variants a, b, and c encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ANXA11
• Entrez:
  • 311(Human)
  • 11744(Mouse)
  • 290527(Rat)