Annexin A1 Antibody (SAIC-13B-19) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Annexin A1 Antibody (SAIC-13B-19) - Azide and BSA Free [NBP3-20092] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-13B-19 (NBP3-20092) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20092, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Western Blot: Annexin A1 Antibody (SAIC-13B-19) - Azide and BSA Free [NBP3-20092] - A431(A) (0.1ug/ml), A549(B) (0.1ug/ml), HeLa(C) (0.1ug/ml), and K562(D) (0.3ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-13B-19 (NBP3-20092), before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Flow Cytometry: Annexin A1 Antibody (SAIC-13B-19) - Azide and BSA Free [NBP3-20092] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-13B-19 (NBP3-20092, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, MS
• Clone: SAIC-13B-19
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Annexin A1 Antibody (SAIC-13B-19) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Annexin A1 Antibody (SAIC-13B-19) - Azide and BSA Free (NBP3-20092) is a recombinant monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Peptide "AAYLQETGKPLDETLK" derived from Annexin A1 conjugated to KLH.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ANXA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Mass Spectrometry
  • Western Blot
• Application Notes: This Ab can be used in immunoMRM.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Annexin A1 Antibody (SAIC-13B-19) - Azide and BSA Free

• Annexin A1
• annexin I (lipocortin I)
• Annexin I
• Annexin-1
• ANX1
• ANXA1
• Calpactin II
• calpactin-2
• chromobindin-9
• Lipocortin I
• LPC1
• LPC1annexin-1
• p35
• Phospholipase A2 inhibitory protein

Background

Annexin I, also known as Annexin A1, belongs to the annexin family of calcium dependent phospholipid binding proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANXA1