Annexin A1 Antibody (686122) [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse Annexin A1 Monoclonal Antibody (Catalog # MAB37701) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Annexin A1 at approximately 45 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat Annexin A1 Monoclonal Antibody (Catalog # MAB37701). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western lane view shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Annexin A1 at approximately 45 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat Annexin A1 Monoclonal Antibody (Catalog # MAB37701). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast parental cell line and Annexin A1 knockout NIH-3T3 cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat Annexin A1 Monoclonal Antibody (Catalog # MAB37701) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Annexin A1 at approximately 38 kDa (as indicated) in the parental NIH-3T3 cell line, but is not detectable in knockout NIH-3T3 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, KO
• Clone: 686122
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Annexin A1 Antibody (686122) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Annexin A1
  Met1-Asn346
  Accession # P04083
• Specificity: Detects human Annexin A1 in direct ELISAs and human, mouse, and rat Annexin A1 in Western blots. In direct ELISAs, approximately 25%‑50% cross-reactivity with recombinant human (rh) Annexin A2 is observed and no cross-reactivity with rhAnnexin A3, A6, or A11 is observed. In Western blots, no cross-reactivity with rhAnnexin A6 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANXA1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Annexin A1 Antibody (686122) [Unconjugated]

• Annexin A1
• annexin I (lipocortin I)
• Annexin I
• Annexin-1
• ANX1
• ANXA1
• Calpactin II
• calpactin-2
• Chromobindin-9
• Lipocortin I
• LPC1
• LPC1annexin-1
• p35
• Phospholipase A2 inhibitory protein

Background

The Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexins have a molecular weight of approximately 35 to 40 kDa and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C‑terminal domain is comprised of four 60‑70 amino acid repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats). The four annexin repeats form a highly alpha -helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner. Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Annexin A1 (ANXA1), also known as annexin I, lipocortin I, and calpactin II, is an ~ 40 kDa protein with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, Annexin A1 may have anti-inflammatory activity. Human Annexin A1 shares 88 and 89% amino acid sequence identity with mouse and rat Annexin A1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Annexin A1 Antibody (MAB37701)(3)

Publications using MAB37701

• Castellani, G;Buccarelli, M;D'Alessandris, QG;Ilari, R;Cappannini, A;Pedini, F;Boe, A;Lulli, V;Parolini, I;Giannetti, S;Biffoni, M;Zappavigna, V;Marziali, G;Pallini, R;Ricci-Vitiani, L; Extracellular vesicles produced by irradiated endothelial or Glioblastoma stem cells promote tumor growth and vascularization modulating tumor microenvironment Cancer cell international 2024-02-12 [PMID: 38347567] (Western Blot, Human)
• GI Perez, MP Bernard, D Vocelle, AA Zarea, NA Saleh, MA Gagea, D Schneider, M Bauzon, T Hermiston, M Kanada Phosphatidylserine-Exposing Annexin A1-Positive Extracellular Vesicles: Potential Cancer Biomarkers Vaccines, 2023-03-13;11(3):. 2023-03-13 [PMID: 36992223] (Western Blot, Human)
• KL Allen, J Cann, W Zhao, N Peterson, M Lazzaro, H Zhong, H Wu, WF Dall'Acqua, MJ Borrok, MM Damschrode, P Tsui, Q Li Upregulation of annexin A1 protein expression in the intratumoral vasculature of human non-small-cell lung carcinoma and rodent tumor models PLoS ONE, 2020-06-04;15(6):e0234268. 2020-06-04 [PMID: 32497150] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ANXA1
• Uniprot:
  • P04083()