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Annexin A1 Antibody (2F1)

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Western Blot: Annexin A1 Antibody (2F1) [NBP2-23485] - WB analysis of ANXA1 in (1) Hela, (2) A549, (3) K562, (4) NIH3T3, (5) C6, and (6) COS7 cell lysate.
Western Blot: Annexin A1 Antibody (2F1) [NBP2-23485] - WB analysis of ANXA1 in (1) Hela, (2) A549, (3) K562, (4) NIH3T3, (5) C6, and (6) COS7 cell lysate.
Immunohistochemistry: Annexin A1 Antibody (2F1) [NBP2-23485] - IHC analysis of paraffin embedded human cervical cancer tissues with DAB staining.
Western Blot: Annexin A1 Antibody (2F1) [NBP2-23485] - WB detection of ANXA1 in human ANXA1 recombinant protein.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
2F1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-23485AF350
Alexa Fluor 532
NBP2-23485AF532
Alexa Fluor 594
NBP2-23485AF594
Alexa Fluor 750
NBP2-23485AF750
DyLight 594
NBP2-23485DL594
FITC
NBP2-23485F
Janelia Fluor 549
NBP2-23485JF549
Janelia Fluor 646
NBP2-23485JF646
100% Guarantee on every product

Annexin A1 Antibody (2F1) Summary

Immunogen
Partial recombinant human Annexin A1 (between residues 100-300) expressed in E. coli [UniProt P04083]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ANXA1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Control
NIH 3T3 Whole Cell Lysate
HeLa Whole Cell Lysate
Annexin A1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP2-23485 in the following applications:
Publications
Read Publication using
NBP2-23485 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.5% protein stabilizer
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for Annexin A1 Antibody (2F1)

  • Annexin A1
  • annexin I (lipocortin I)
  • Annexin I
  • Annexin-1
  • ANX1
  • ANXA1
  • Calpactin II
  • calpactin-2
  • chromobindin-9
  • Lipocortin I
  • LPC1
  • LPC1annexin-1
  • p35
  • Phospholipase A2 inhibitory protein

Background

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Cdk5 Antibody (4E4) [NBP2-37602] - Analysis using CDK5 mouse mAb against Hela (1), K562 (2), PC-12 (3) and Cos7 (4) cell lysate.
Immunocytochemistry/Immunofluorescence: Cdk5 Antibody (4E4) [NBP2-37602] - Analysis of GC7901 cells using CDK5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Cdk5 Antibody (4E4) [NBP2-37602] - Analysis of cerebellum tissues (left) and brain tissues (right) using CDK5 mouse mAb with DAB staining.
Species: Hu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
ERK2 Antibody
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NBP2-67360
Western Blot: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of ERK2 on different lysates using anti-ERK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK2 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
p63/TP73L Antibody
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p63/TP73L was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(3 Reviews)
Species: Hu
Applications: WB, IHC, ICC
CDK5 Activator 1
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AF7250
CDK5 Activator 1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human CDK5 Activator 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7250) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent‑Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter­stained with hematoxylin (blue). Specific staining was localized to nuclei in neuronal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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IL-9
409-ML/CF
IL-9
(6 Publications)
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(JF0955)
NBP2-67117
Western Blot: RPSA Antibody (JF0955) [NBP2-67117] - Analysis of RPSA on different lysates using anti-RPSA antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: HepG2 Lane 3: A431
Immunocytochemistry/Immunofluorescence: RPSA Antibody (JF0955) [NBP2-67117] - Staining RPSA in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: RPSA Antibody (JF0955) [NBP2-67117] - Analysis of paraffin-embedded mouse kidney tissue using anti-RPSA antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
GEF-H1 Antibody
NBP2-21577
Western Blot: GEF-H1 Antibody [NBP2-21577] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: GEF-H1 Antibody [NBP2-21577] - HeLa cells were fixed in -20C 100% MeOH for 5 min. Green: ARHGEF2 protein stained by GEF-H1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Frozen: GEF-H1 Antibody [NBP2-21577] - Frozen-sectioned mouse mouse cerebellum. Green: GEF-H1 stained by GEF-H1 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD
EBNA1BP2 Antibody
NBP1-84022
Western Blot: EBNA1BP2 Antibody [NBP1-84022] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: EBNA1BP2 Antibody [NBP1-84022] - Staining of human cell line A-431 shows localization to nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: EBNA1BP2 Antibody [NBP1-84022] - Staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.
(1 Publication)
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Applications: WB, ICC/IF, IHC, IHC-P
LANCL1 Antibody
NBP1-81796
Western Blot: LANCL1 Antibody [NBP1-81796] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: LANCL1 Antibody [NBP1-81796] - Immunofluorescent staining of human cell line A-431 shows localization to microtubules.
Immunohistochemistry-Paraffin: LANCL1 Antibody [NBP1-81796] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.
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p40 Antibody
NBP1-32956
Western Blot: p40 (p63 delta) Antibody [NBP1-32956] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: p40 (p63 delta) Antibody [NBP1-32956] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: p40 (p63 delta) Antibody [NBP1-32956] - A549 xenograft, using p40 antibody at 1:100 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
PSMD7 Antibody
NBP2-38780
Western Blot: PSMD7 Antibody [NBP2-38780] - Analysis using Anti-PSMD7 antibody NBP2-38780 (A) shows similar pattern to independent antibody NBP2-38612 (B).
Immunocytochemistry/Immunofluorescence: PSMD7 Antibody [NBP2-38780] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: PSMD7 Antibody [NBP2-38780] - Staining of human kidney.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Annexin A2 Antibody
NBP1-31310
Knockout Validated: Annexin A2 Antibody [NBP1-31310] - Wild-type (WT) and Annexin II knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody [N1], N-term. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Annexin A2 Antibody [NBP1-31310] - Analysis. Non-transfected (-) and human Annexin I or Annexin II-transfected (+) whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody diluted at 1:1000.
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TNF-alpha
[Unconjugated]
210-TA
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Western Blot: TPPP/p25 Antibody [NBP2-34031] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral cortex tissue.
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[Unconjugated]
1887-ML
IL-23A/IL-23 P19 [Unconjugated]
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Lipocalin-2/NGAL
Antibody [Unconjugate ...
AF1857
Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected forLipocalin‑2/NGAL at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Lipocalin‑2/NGAL was detected in perfusion fixed frozen sections of mouse mammary gland using Goat Anti-Mouse Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular areas in ducts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Lipocalin‑2/NGAL at approximately 37 kDa (as indicated) using 5 µg/mL of Goat Anti-MouseLipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1857) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
(4 Reviews)
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TMED9 Antibody
NBP1-31650
Western Blot: TMED9 Antibody [NBP1-31650] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:5000.
Immunohistochemistry-Paraffin: TMED9 Antibody [NBP1-31650] - Sample: Paraffin-embedded mouse duodenum. TMED9 antibody diluted at 1:500.
Immunohistochemistry-Paraffin: TMED9 Antibody [NBP1-31650] - Paraffin-embedded DLD-1 xenograft, using antibody at 1:500 dilution.
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IFN-gamma
[Unconjugated]
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1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
(13 Reviews)
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PLA2G1B Antibody
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Immunocytochemistry/Immunofluorescence: PLA2G1B Antibody [NBP2-32569] - Staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: PLA2G1B Antibody [NBP2-32569] - Staining in human pancreas and skeletal muscle tissues using anti-PLA2G1B antibody. Corresponding PLA2G1B RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PLA2G1B Antibody [NBP2-32569] - Staining of human pancreas shows high expression.
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Annexin A1
Antibody (2F1)
NBP2-23485
Annexin A1 Antibody (2F1)
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, IHC, IHC-P

Publications for Annexin A1 Antibody (NBP2-23485)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IHC.


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Review for Annexin A1 Antibody (NBP2-23485) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-23485:
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Images Ratings Applications Species Date Details
Western Blot Annexin A1 NBP2-23485
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reviewed by:
Anonymous
 WB Human 10/02/2019
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Summary

ApplicationWestern Blot
Sample Tested Hela whole cell lysate
SpeciesHuman
LotA-1

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Control Lysate(s)

NIH 3T3 Whole Cell Lysate
HeLa Whole Cell Lysate
Annexin A1 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-23485AF350
Alexa Fluor 532 NBP2-23485AF532
Alexa Fluor 594 NBP2-23485AF594
Alexa Fluor 750 NBP2-23485AF750
DyLight 594 NBP2-23485DL594
FITC NBP2-23485F
Janelia Fluor 549 NBP2-23485JF549
Janelia Fluor 646 NBP2-23485JF646
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Western Blot Annexin A1 NBP2-23485
Enlarge
 Anonymous
10/02/2019
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol ANXA1
Entrez
Uniprot