Annexin A1 Antibody (2F1)

Images

 
Western Blot: Annexin A1 Antibody (2F1) [NBP2-23485] - WB analysis of ANXA1 in (1) Hela, (2) A549, (3) K562, (4) NIH3T3, (5) C6, and (6) COS7 cell lysate.
Immunohistochemistry: Annexin A1 Antibody (2F1) [NBP2-23485] - IHC analysis of paraffin embedded human cervical cancer tissues with DAB staining.
Western Blot: Annexin A1 Antibody (2F1) [NBP2-23485] - WB detection of ANXA1 in human ANXA1 recombinant protein.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
2F1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Annexin A1 Antibody (2F1) Summary

Immunogen
Partial recombinant human Annexin A1 (between residues 100-300) expressed in E. coli [UniProt P04083]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ANXA1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Application Notes
Use in ICC/IF was reported in scientific literature (PMID: 30045979).
Control
NIH 3T3 Whole Cell Lysate
HeLa Whole Cell Lysate
Annexin A1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP2-23485 in the following applications:

Publications
Read Publication using
NBP2-23485 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.5% protein stabilizer
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for Annexin A1 Antibody (2F1)

  • Annexin A1
  • annexin I (lipocortin I)
  • Annexin I
  • Annexin-1
  • ANX1
  • ANXA1
  • Calpactin II
  • calpactin-2
  • chromobindin-9
  • Lipocortin I
  • LPC1
  • LPC1annexin-1
  • p35
  • Phospholipase A2 inhibitory protein

Background

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for Annexin A1 Antibody (NBP2-23485)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IHC.


Filter By Application
ICC/IF
(1)
IHC
(1)
All Applications
Filter By Species
Human
(1)
All Species

Review for Annexin A1 Antibody (NBP2-23485) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-23485:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Annexin A1 NBP2-23485
Enlarge
5
reviewed by:
Anonymous
WB Human 10/02/2019
View

Summary

ApplicationWestern Blot
Sample Tested Hela whole cell lysate
SpeciesHuman
LotA-1

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Annexin A1 Antibody (NBP2-23485) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-23485AF350
Alexa Fluor 488 NBP2-23485AF488
Alexa Fluor 532 NBP2-23485AF532
Alexa Fluor 594 NBP2-23485AF594
Alexa Fluor 750 NBP2-23485AF750
DyLight 594 NBP2-23485DL594
FITC NBP2-23485F
Janelia Fluor 549 NBP2-23485JF549
Janelia Fluor 646 NBP2-23485JF646

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Bioinformatics Tool for Annexin A1 Antibody (NBP2-23485)

Discover related pathways, diseases and genes to Annexin A1 Antibody (NBP2-23485). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Annexin A1 Antibody (NBP2-23485)

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PTMs for Annexin A1 Antibody (NBP2-23485)

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Research Areas for Annexin A1 Antibody (NBP2-23485)

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Blogs on Annexin A1

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
10/02/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol ANXA1
Entrez
Uniprot