Annexin A1 Antibody (2F1)

Western Blot: Annexin A1 Antibody (2F1) [NBP2-23485] - WB analysis of ANXA1 in (1) Hela, (2) A549, (3) K562, (4) NIH3T3, (5) C6, and (6) COS7 cell lysate.

Immunohistochemistry: Annexin A1 Antibody (2F1) [NBP2-23485] - IHC analysis of paraffin embedded human cervical cancer tissues with DAB staining.

Western Blot: Annexin A1 Antibody (2F1) [NBP2-23485] - WB detection of ANXA1 in human ANXA1 recombinant protein.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Annexin A1 Antibody (2F1) Summary

• Immunogen: Partial recombinant human Annexin A1 (between residues 100-300) expressed in E. coli [UniProt P04083]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANXA1
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 30045979)
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Control:

  NIH 3T3 Whole Cell Lysate
  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.5% protein stabilizer
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for Annexin A1 Antibody (2F1)

• Annexin A1
• annexin I (lipocortin I)
• Annexin I
• Annexin-1
• ANX1
• ANXA1
• Calpactin II
• calpactin-2
• chromobindin-9
• Lipocortin I
• LPC1
• LPC1annexin-1
• p35
• Phospholipase A2 inhibitory protein

Background

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Annexin A1 Antibody (NBP2-23485)(2)

Publications using NBP2-23485

• Xu J, Yu C, Luo J, et al. The role and mechanism of the annexin A1 peptide Ac2-26 in rats with cardiopulmonary bypass lung injury Basic & clinical pharmacology & toxicology 2021-01-17 [PMID: 33455036]
• Iglesias-Bartolome R, Uchiyama A, Molinolo AA et al. Transcriptional signature primes human oral mucosa for rapid wound healing. Sci Transl Med. 2018-07-25 [PMID: 30045979] (ICC/IF, IF/IHC, Human)

Review for Annexin A1 Antibody (NBP2-23485) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/02/2019

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: A-1

Bioinformatics

• Gene Symbol: ANXA1
• Entrez:
  • 301(Human)
  • 16952(Mouse)
• Uniprot:
  • P04083()
  • P10107(Mouse)