![Immunohistochemistry-Paraffin: ANKRD17 Antibody [NB100-86996] - Human prostate carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).](http://images.novusbio.com/fullsize/ANKRD17-Antibody-Immunohistochemistry-Paraffin-NB100-86996-img0003.jpg "Immunohistochemistry-Paraffin: ANKRD17 Antibody [NB100-86996] - Human prostate carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | IHC, IP, WB (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit ANKRD17 Antibody - BSA Free (NB100-86996) is a polyclonal antibody validated for use in IHC and IP. Anti-ANKRD17 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1550 and 1600 of human ankyrin repeat domain 17 using the numbering given in entry NP_115593.3 (GeneID 26057). |
| Predicted Species | Mouse (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ANKRD17 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Western Blot Not recommended. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
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![Simple Western alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](https://images.novusbio.com/images2/16253.jpg)
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| Publication using NB100-86996 | Applications | Species |
|---|---|---|
| Ray D, Hogarth CA, Evans EB et al. Experimental validation of Ankrd17 and Anapc10, two novel meiotic genes predicted by computational models in mice. Biol Reprod 2012-04-01 [PMID: 22190705] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ANKRD17 |
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| Uniprot |
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![Immunoprecipitation: ANKRD17 Antibody [NB100-86996] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-86996 used for IP at 3 mcg/mg lysate. ANKRD17 was also immunoprecipitated by rabbit anti-ANKRD17 antibody NB100-86995, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/ANKRD17-Antibody-Western-Blot-NB100-86996-img0002.jpg "Immunoprecipitation: ANKRD17 Antibody [NB100-86996] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-86996 used for IP at 3 mcg/mg lysate. ANKRD17 was also immunoprecipitated by rabbit anti-ANKRD17 antibody NB100-86995, which recognizes an upstream epitope.")
![Holo-Transferrin [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Holo-Transferrin [Unconjugated]")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")