Anillin Antibody

Immunocytochemistry/Immunofluorescence: Anillin Antibody [NB100-1314] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: Anillin Antibody [NB100-1314] - 10ug/ml staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Flow Cytometry: Anillin Antibody [NB100-1314] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Bv
• Applications: ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Anillin Antibody Summary

• Immunogen: Peptide with sequence WQPDACYKPIGKP corresponding to C-Terminus according to NP_061155.2.
• Predicted Species: Mouse (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ANLN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 3 - 10 ug/ml
  • Immunohistochemistry-Paraffin 3 - 10 ug/ml
  • Peptide ELISA Detection limit 1:64000
• Application Notes: WB: Preliminary experiments gave an approx. 75 kDa band in mouse brain lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 124 kDa band according to NP_061155.2. The 75 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows staining of nuclei in some cells of renal tubules.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Anillin Antibody

• actin-binding protein anillin
• anillin (Drosophila Scraps homolog), actin binding protein
• ANILLIN
• anillin, actin binding protein (scraps homolog, Drosophila)
• anillin, actin binding protein
• DKFZp779A055
• Drosophila Scraps homolog
• scra
• Scraps

Background

Anillin is an actin-binding protein that can bind septins and is a component of the cytokinetic ring. Anillin is expressed ubiquitously but with variable levels of expression, being highest in the central nervous system. Anillin is overexpressed in diverse common human tumors, but not simply as a consequence of being a proliferation marker. Anillin may have potential as a novel biomarker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Anillin Antibody (NB100-1314)(1)

Publication using NB100-1314

• Oegema K, Savoian MS, Mitchison TJ, Field CM. Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis. J Cell Biol 2000-08-07 [PMID: 10931866]

Bioinformatics

• Gene Symbol: ANLN
• Entrez:
  • 54443(Human)
  • 68743(Mouse)
• Uniprot:
  • NP_061155.2(Human)