Anillin Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Anillin Antibody [NB100-1314] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Immunohistochemistry-Paraffin: Anillin Antibody [NB100-1314] - 10ug/ml staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Flow Cytometry: Anillin Antibody [NB100-1314] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Anillin Antibody Summary

Immunogen
Peptide with sequence WQPDACYKPIGKP corresponding to C-Terminus according to NP_061155.2.
Predicted Species
Mouse (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ANLN
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 3 - 10 ug/ml
  • Immunohistochemistry-Paraffin 3 - 10 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Preliminary experiments gave an approx. 75 kDa band in mouse brain lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 124 kDa band according to NP_061155.2. The 75 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows staining of nuclei in some cells of renal tubules.
Publications
Read Publication using NB100-1314.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Anillin Antibody

  • actin-binding protein anillin
  • anillin (Drosophila Scraps homolog), actin binding protein
  • ANILLIN
  • anillin, actin binding protein (scraps homolog, Drosophila)
  • anillin, actin binding protein
  • DKFZp779A055
  • Drosophila Scraps homolog
  • scra
  • Scraps

Background

Anillin is an actin-binding protein that can bind septins and is a component of the cytokinetic ring. Anillin is expressed ubiquitously but with variable levels of expression, being highest in the central nervous system. Anillin is overexpressed in diverse common human tumors, but not simply as a consequence of being a proliferation marker. Anillin may have potential as a novel biomarker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-1314
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Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA

Publications for Anillin Antibody (NB100-1314)(1)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ANLN
Entrez
Uniprot