Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) - BSA Free

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Biological Strategies: Western Blot: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Protein expressions of intrarenal RAS components in two groups of mice. Western blot analysis for protein ...read more
Biological Strategies: Immunohistochemistry: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Protein expressions of intrarenal RAS components in two groups of mice. (A) Immunohistochemical ...read more
Western Blot: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Evaluation of angiotensis II expression in untreated UUO rat kidneys and UUO rat kidneys treated with Ang-(1-7).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clone
Ang II E7 (BGN/KA/4L)
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) - BSA Free Summary

Immunogen
Peptide sequence corresponding to angiotensin II, conjugated to a proprietary molecular weight carrier. Peptide sequence: DRVYIHPF
Specificity
This product reacts with Angiotensin II and III. Negligible reactivity was observed with Angiotensin I and Angiotensinogen.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
AGT
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
Use in Immunohistochemistry on paraffin sections was reported in a scientific publication (PMID: 23570453). Use in Western Blot was reported in a scientific publication (PMID: 23800500).
Publications
Read Publications using
NB100-62346 in the following applications:

Reactivity Notes

Based on sequence similarity this antibody is expected to react with Sheep, Pig, and Horse. Mouse reactivity reported in a scientific publication (PMID: 23570453). Rat reactivity reported in a scientific publication (PMID: 23800500).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) - BSA Free

  • AGT angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
  • angiotensin I
  • angiotensin II
  • ANHU
  • SERPINA8

Background

Angiotensin is a polypeptide vasopressor hormone formed by the catalytic action of renin, which is released mainly by the kidneys in response to low blood volume or low sodium content in the body. The juxtaglomerular cells release renin when juxtaglomerular apparatus decreases in the kidneys. Renin (called angiotensinogenase) is an enzyme of the hydrolase class that catalyzes cleavage of the leucine leucine bond in angiotensinogen (a serum globulin) to generate angiotensin I in the liver. Angiotensin I acts mainly as a precursor to angiotensin II, which is a vasopressor and stimulator of aldosterone secretion by the adrenal cortex, and functions also as a neurotransmitter. Angiotensin II is formed by catalytic action of the hydrolase called peptidyl dipeptidase A on C terminal oligopeptide (angiotensin I). In case of the cleavage of angiotensin I to the activated angiotensin II, it is called angiotensin converting enzyme (ACE); in case of the cleavage and inactivation of kinins, it is called kininase II (also called dipeptidyl carboxypeptidase I). The renin-angiotensin system plays an important role in the regulation of blood volume and blood pressure, stimulates antidiuretic hormone and aldosterone production, and the hypothalamus to activate the thirst reflex, but is crucial in the pathogenesis of hypertension patients due to blood vessels constrict, retention of fluid and raising blood pressure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiotensin II/III Antibody (NB100-62346)(17)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ELISA, ICC/IF, IF/IHC, IHC-P, WB.


Filter By Application
ELISA
(1)
ICC/IF
(1)
IF/IHC
(2)
IHC-P
(4)
WB
(10)
All Applications
Filter By Species
Human
(3)
Mouse
(6)
Rat
(7)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB100-62346 Applications Species
Wu Q, Zhou S, Xu D et al. The CXCR4-AT1 axis plays a vital role in glomerular injury via mediating the crosstalk between podocyte and mesangial cell Translational research : the journal of laboratory and clinical medicine 2023-09-09 [PMID: 37696390]
Meister ML, Najjar RS, Danh JP et al. Berry consumption mitigates the hypertensive effects of a high-fat, high-sucrose diet via attenuation of renal and aortic AT1R expression resulting in improved endothelium-derived NO bioavailability The Journal of nutritional biochemistry 2022-11-23 [PMID: 36435288] (WB, Mouse, Human) WB Mouse, Human
Wang W, Su L, Wang Y et al. Endothelial Cells Mediated by UCP2 Control the Neurogenic-to-Astrogenic Neural Stem Cells Fate Switch During Brain Development Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022-04-30 [PMID: 35488517] (WB, Human) WB Human
Dang Z, Su S, Jin G, et al. Tsantan Sumtang attenuated chronic hypoxia-induced right ventricular structure remodeling and fibrosis by equilibrating local ACE-AngII-AT1R/ACE2-Ang1-7-Mas axis in rat J Ethnopharmacol 2019-12-17 [PMID: 31862407] (IF/IHC, Rat) IF/IHC Rat
Sukketsiri W, Hoshino K, Kugo H et al. Isoflavone Ameliorated Oxidative Stress and Vascular Damages in Nicotine-Administrated Mice J Oleo Sci 2019-12-01 [PMID: 31735744] (IHC-P, Mouse) IHC-P Mouse
Bae EH, Kim IJ, Choi HS et al. Tumor necrosis factor A-converting enzyme inhibitor attenuates lipopolysaccharide-induced reactive oxygen species and mitogen-activated protein kinase expression in human renal proximal tubule epithelial cells. Korean J. Physiol. Pharmacol. 2018-03-01 [PMID: 29520166] (WB, Mouse) WB Mouse
Dornhoffer, J R, Wei, T Et al. The expression of renin-angiotensin-aldosterone axis components in infantile hemangioma tissue and the impact of propranolol treatment. Pediatr Res 2017-07-01 [PMID: 28376078] (ICC/IF, Rat) ICC/IF Rat
Watanabe R, Suzuki JI, Wakayama K et al. A peptide vaccine targeting angiotensin II attenuates the cardiac dysfunction induced by myocardial infarction. Sci Rep. 2017-03-07 [PMID: 28266578] (WB, Rat) WB Rat
Kim CS, Kim IJ, Bae EH et al. Angiotensin-(1-7) Attenuates Kidney Injury Due to Obstructive Nephropathy in Rats. PLoS ONE. 2015-11-11 [PMID: 26556707] (WB, Rat) WB Rat
Okamoto K, Fujii H, Goto S et al. Influence of Coexisting Chronic Kidney Disease and Left Ventricular Hypertrophy on Changes in FGF23 and the Renin-Angiotensin-Aldosterone-System Research Square 2021-06-24 (IHC-P) IHC-P
Show All 17 Publications.

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FAQs for Angiotensin II/III Antibody (NB100-62346). (Showing 1 - 1 of 1 FAQs).

  1. According to the product datasheet, NB100-62346 indicates Angiotensin II/III antibody.  However, the Angiotensin II protein (NBP1-99199) which you recommand to use to do the preabsorption test is Serpin A8/Angiotensinogen partial recombinant protein (38 kDa).  Which one was used to produce Angiotensin II/III antibody (NB100-62346), angiotensin II or angiotensinogen partial recombinant protein ?
    • Product NB100-62346 was made to the angiotensin II protein (aa34-41), which is a partial recombinant protein of the angiotensinogen full length protein (aa 1-485). You can use protein NBP1-99199 to perform a pre-absorption test since it is the same amino acids as the antigen used to make NB100-62346.

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Bioinformatics

Gene Symbol AGT
Entrez
Uniprot