Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L))

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Western Blot: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Evaluation of angiotensis II expression in untreated UUO rat kidneys and UUO rat kidneys treated with Ang-(1-7).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
Ang II E7 (BGN/KA/4L)
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) Summary

Immunogen
Peptide sequence corresponding to angiotensin II, conjugated to a proprietary molecular weight carrier. Peptide sequence: DRVYIHPF
Specificity
This product reacts with Angiotensin II and III. Negligible reactivity was observed with Angiotensin I and Angiotensinogen.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
AGT
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Use in Immunohistochemistry on paraffin sections was reported in a scientific publication (PMID: 23570453). Use in Western Blot was reported in a scientific publication (PMID: 23800500).
Publications
Read Publications using
NB100-62346 in the following applications:

Reactivity Notes

Based on sequence similarity this antibody is expected to react with Sheep, Pig, and Horse. Mouse reactivity reported in a scientific publication (PMID: 23570453). Rat reactivity reported in a scientific publication (PMID: 23800500).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L))

  • AGT angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
  • angiotensin I
  • angiotensin II
  • ANHU
  • SERPINA8

Background

Angiotensin is a polypeptide vasopressor hormone formed by the catalytic action of renin, which is released mainly by the kidneys in response to low blood volume or low sodium content in the body. The juxtaglomerular cells release renin when juxtaglomerular apparatus decreases in the kidneys. Renin (called angiotensinogenase) is an enzyme of the hydrolase class that catalyzes cleavage of the leucine leucine bond in angiotensinogen (a serum globulin) to generate angiotensin I in the liver. Angiotensin I acts mainly as a precursor to angiotensin II, which is a vasopressor and stimulator of aldosterone secretion by the adrenal cortex, and functions also as a neurotransmitter. Angiotensin II is formed by catalytic action of the hydrolase called peptidyl dipeptidase A on C terminal oligopeptide (angiotensin I). In case of the cleavage of angiotensin I to the activated angiotensin II, it is called angiotensin converting enzyme (ACE); in case of the cleavage and inactivation of kinins, it is called kininase II (also called dipeptidyl carboxypeptidase I). The renin-angiotensin system plays an important role in the regulation of blood volume and blood pressure, stimulates antidiuretic hormone and aldosterone production, and the hypothalamus to activate the thirst reflex, but is crucial in the pathogenesis of hypertension patients due to blood vessels constrict, retention of fluid and raising blood pressure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Angiotensin II/III Antibody (NB100-62346)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ELISA, IHC-P, WB.


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ELISA
(1)
IHC-P
(3)
WB
(8)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
Rat
(5)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB100-62346 Applications Species
Jang HS, Kim JI, Kim J, Park JW. Angiotensin II Removes Kidney Resistance Conferred by Ischemic Preconditioning. BioMed Research Internationa. 2014 Aug 28 (WB, IHC-P, Mouse) WB, IHC-P Mouse
Su YY, Li HM, Yan ZX et al. Renin-angiotensin system activation and imbalance of matrix metalloproteinase-9/tissue inhibitor of matrix metalloproteinase-1 in cold-induced stroke Life Sci. 2019 Jun 11 [PMID: 31200003] (WB, IHC-P, Rat) WB, IHC-P Rat
Bae EH, Kim IJ, Choi HS et al. Tumor necrosis factor A-converting enzyme inhibitor attenuates lipopolysaccharide-induced reactive oxygen species and mitogen-activated protein kinase expression in human renal proximal tubule epithelial cells. Korean J. Physiol. Pharmacol. Mar 1 2018 [PMID: 29520166] (WB, Mouse) WB Mouse
Watanabe R, Suzuki JI, Wakayama K et al. A peptide vaccine targeting angiotensin II attenuates the cardiac dysfunction induced by myocardial infarction. Sci Rep. Mar 7 2017 [PMID: 28266578] (WB, Rat) WB Rat
Kim CS, Kim IJ, Bae EH et al. Angiotensin-(1-7) Attenuates Kidney Injury Due to Obstructive Nephropathy in Rats. PLoS ONE. 2015 Nov 11 [PMID: 26556707] (WB, Rat) WB Rat
Mori K, Noguchi M, Tobu S et al. Age-related changes in bladder function with altered angiotensin II receptor mechanisms in rats. Neurourol. Urodyn. 2015 Aug 07 [PMID: 26250880] (WB, Rat) WB Rat
Wang H, Wang J, Guo C et al. Renal denervation attenuates progression of atherosclerosis in apolipoprotein E-deficient mice independent of blood pressure lowering. Hypertension. 2015 Apr 01 [PMID: 25646301] (ELISA, Mouse) ELISA Mouse
Ma KL, Ni J, Wang CX et al. Interaction of RAS Activation and Lipid Disorders Accelerates the Progression of Glomerulosclerosis. Int J Med Sci. 2013 Sep 18 [PMID: 24151433] (WB, Human) WB Human
Li C, Li X, Shen Q et al. Critical Role of Matrix Metalloproteinase-9 in Acute Cold Exposure-Induced Stroke in Renovascular Hypertensive Rats. J Stroke Cerebrovasc Dis 2013 Jun 22 [PMID: 23800500] (WB, Rat) WB Rat
Ni J, Ma KL, Wang CX et al. Activation of renin-angiotensin system is involved in dyslipidemia-mediated renal injuries in apolipoprotein E knockout mice and HK-2 cells. Lipids Health Dis 2013 Apr 9 [PMID: 23570453] (IHC-P, Mouse) IHC-P Mouse

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Angiotensin II/III Antibody (NB100-62346). (Showing 1 - 1 of 1 FAQs).

  1. According to the product datasheet, NB100-62346 indicates Angiotensin II/III antibody.  However, the Angiotensin II protein (http://www.novusbio.com/Serpin-A8-Angiotensinogen-Protein_NBP1-99199.html.) which you recommand to use to do the preabsorption test is Serpin A8/Angiotensinogen partial recombinant protein (38 kDa).  Which one was used to produce Angiotensin II/III antibody (NB100-62346), angiotensin II or angiotensinogen partial recombinant protein ?
    • Product NB100-62346 was made to the angiotensin II protein (aa34-41), which is a partial recombinant protein of the angiotensinogen full length protein (aa 1-485). You can use protein NBP1-99199 to perform a pre-absorption test since it is the same amino acids as the antigen used to make NB100-62346.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-62346AF350
Alexa Fluor 405 NB100-62346AF405
Alexa Fluor 488 NB100-62346AF488
Alexa Fluor 532 NB100-62346AF532
Alexa Fluor 594 NB100-62346AF594
Alexa Fluor 647 NB100-62346AF647
Alexa Fluor 700 NB100-62346AF700
Alexa Fluor 750 NB100-62346AF750
Biotin NB100-62346B
DyLight 350 NB100-62346UV
DyLight 405 NB100-62346V
DyLight 488 NB100-62346G
DyLight 550 NB100-62346R
DyLight 650 NB100-62346C
DyLight 680 NB100-62346FR
DyLight 755 NB100-62346IR
FITC NB100-62346F
HRP NB100-62346H
Janelia Fluor 549 NB100-62346JF549
Janelia Fluor 646 NB100-62346JF646

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Bioinformatics

Gene Symbol AGT
Entrez
Uniprot