Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) - BSA Free

Western Blot: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Protein expressions of intrarenal RAS components in two groups of mice. Western blot analysis for protein expressions of intrarenal RAS components in the two groups of mice. Image collected and cropped by CiteAb from the following publication (lipidworld.biomedcentral.com/articles/10.1186/1476-511X-12-49), licensed under a CC-BY licence.

Immunohistochemistry: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Protein expressions of intrarenal RAS components in two groups of mice. (A) Immunohistochemical analysis of intrarenal RAS expression position in the two groups of mice. AGT immunoreactivity in the proximal tubular cells, Ang II immunoreactivity in both glomerular and tubular cells, renin immunoreactivity in juxtaglomerular apparatus cells, ACE immunoreactivity in brush border membranes of proximal tubules as well as AT1 and AT2 immunoreactivity in the proximal tubules were increased in the HF group when compared to the control group. Image collected and cropped by CiteAb from the following publication (lipidworld.biomedcentral.com/articles/10.1186/1476-511X-12-49), licensed under a CC-BY licence.

Western Blot: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Evaluation of angiotensis II expression in untreated UUO rat kidneys and UUO rat kidneys treated with Ang-(1-7).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC, IHC-P
• Clone: Ang II E7 (BGN/KA/4L)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) - BSA Free Summary

• Immunogen: Peptide sequence corresponding to angiotensin II, conjugated to a proprietary molecular weight carrier. Peptide sequence: DRVYIHPF
• Specificity: This product reacts with Angiotensin II and III. Negligible reactivity was observed with Angiotensin I and Angiotensinogen.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: AGT
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Use in Immunohistochemistry on paraffin sections was reported in a scientific publication (PMID: 23570453). Use in Western Blot was reported in a scientific publication (PMID: 23800500).

Reactivity Notes

Based on sequence similarity this antibody is expected to react with Sheep, Pig, and Horse. Mouse reactivity reported in a scientific publication (PMID: 23570453). Rat reactivity reported in a scientific publication (PMID: 23800500).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) - BSA Free

• AGT angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• angiotensin I
• angiotensin II
• ANHU
• SERPINA8

Background

Angiotensin is a polypeptide vasopressor hormone formed by the catalytic action of renin, which is released mainly by the kidneys in response to low blood volume or low sodium content in the body. The juxtaglomerular cells release renin when juxtaglomerular apparatus decreases in the kidneys. Renin (called angiotensinogenase) is an enzyme of the hydrolase class that catalyzes cleavage of the leucine leucine bond in angiotensinogen (a serum globulin) to generate angiotensin I in the liver. Angiotensin I acts mainly as a precursor to angiotensin II, which is a vasopressor and stimulator of aldosterone secretion by the adrenal cortex, and functions also as a neurotransmitter. Angiotensin II is formed by catalytic action of the hydrolase called peptidyl dipeptidase A on C terminal oligopeptide (angiotensin I). In case of the cleavage of angiotensin I to the activated angiotensin II, it is called angiotensin converting enzyme (ACE); in case of the cleavage and inactivation of kinins, it is called kininase II (also called dipeptidyl carboxypeptidase I). The renin-angiotensin system plays an important role in the regulation of blood volume and blood pressure, stimulates antidiuretic hormone and aldosterone production, and the hypothalamus to activate the thirst reflex, but is crucial in the pathogenesis of hypertension patients due to blood vessels constrict, retention of fluid and raising blood pressure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiotensin II/III Antibody (NB100-62346)(16)

Publications using NB100-62346

• Meister ML, Najjar RS, Danh JP et al. Berry consumption mitigates the hypertensive effects of a high-fat, high-sucrose diet via attenuation of renal and aortic AT1R expression resulting in improved endothelium-derived NO bioavailability The Journal of nutritional biochemistry 2022-11-23 [PMID: 36435288] (WB, Human, Mouse)
• Wang W, Su L, Wang Y et al. Endothelial Cells Mediated by UCP2 Control the Neurogenic-to-Astrogenic Neural Stem Cells Fate Switch During Brain Development Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022-04-30 [PMID: 35488517] (WB, Human)
• Dang Z, Su S, Jin G, et al. Tsantan Sumtang attenuated chronic hypoxia-induced right ventricular structure remodeling and fibrosis by equilibrating local ACE-AngII-AT1R/ACE2-Ang1-7-Mas axis in rat J Ethnopharmacol 2019-12-17 [PMID: 31862407] (IHC, Rat)
• Sukketsiri W, Hoshino K, Kugo H et al. Isoflavone Ameliorated Oxidative Stress and Vascular Damages in Nicotine-Administrated Mice J Oleo Sci 2019-12-01 [PMID: 31735744] (IHC-P, Mouse)
• Su YY, Li HM, Yan ZX et al. Renin-angiotensin system activation and imbalance of matrix metalloproteinase-9/tissue inhibitor of matrix metalloproteinase-1 in cold-induced stroke Life Sci. 2019-06-11 [PMID: 31200003] (IHC-P, WB, Rat)
• Bae EH, Kim IJ, Choi HS et al. Tumor necrosis factor A-converting enzyme inhibitor attenuates lipopolysaccharide-induced reactive oxygen species and mitogen-activated protein kinase expression in human renal proximal tubule epithelial cells. Korean J. Physiol. Pharmacol. 2018-03-01 [PMID: 29520166] (WB, Mouse)
• Watanabe R, Suzuki JI, Wakayama K et al. A peptide vaccine targeting angiotensin II attenuates the cardiac dysfunction induced by myocardial infarction. Sci Rep. 2017-03-07 [PMID: 28266578] (WB, Rat)
• Kim CS, Kim IJ, Bae EH et al. Angiotensin-(1-7) Attenuates Kidney Injury Due to Obstructive Nephropathy in Rats. PLoS ONE. 2015-11-11 [PMID: 26556707] (WB, Rat)
• Mori K, Noguchi M, Tobu S et al. Age-related changes in bladder function with altered angiotensin II receptor mechanisms in rats. Neurourol. Urodyn. 2015-08-07 [PMID: 26250880] (WB, Rat)
• Wang H, Wang J, Guo C et al. Renal denervation attenuates progression of atherosclerosis in apolipoprotein E-deficient mice independent of blood pressure lowering. Hypertension. 2015-04-01 [PMID: 25646301] (ELISA, Mouse)
• Angiotensin II Removes Kidney Resistance Conferred by Ischemic Preconditioning. Jang HS, Kim JI, Kim J Biomed Res Int [PMID: 25243156] (IHC, WB, Mouse)
• Ma KL, Ni J, Wang CX et al. Interaction of RAS Activation and Lipid Disorders Accelerates the Progression of Glomerulosclerosis. Int J Med Sci. 2013-09-18 [PMID: 24151433] (WB, Human)
• Li C, Li X, Shen Q et al. Critical Role of Matrix Metalloproteinase-9 in Acute Cold Exposure-Induced Stroke in Renovascular Hypertensive Rats. J Stroke Cerebrovasc Dis 2013-06-22 [PMID: 23800500] (WB, Rat)
• Ni J, Ma KL, Wang CX et al. Activation of renin-angiotensin system is involved in dyslipidemia-mediated renal injuries in apolipoprotein E knockout mice and HK-2 cells. Lipids Health Dis 2013-04-09 [PMID: 23570453] (IHC-P, Mouse)
• Okamoto K, Fujii H, Goto S et al. Influence of Coexisting Chronic Kidney Disease and Left Ventricular Hypertrophy on Changes in FGF23 and the Renin-Angiotensin-Aldosterone-System Research Square 2021-06-24 (IHC-P)
• Dornhoffer, J R, Wei, T Et al. The expression of renin-angiotensin-aldosterone axis components in infantile hemangioma tissue and the impact of propranolol treatment. Pediatr Res 2017-07-01 [PMID: 28376078] (ICC/IF, Rat)

FAQs for Angiotensin II/III Antibody (NB100-62346). (Showing 1 - 1 of 1 FAQs).

1. According to the product datasheet, NB100-62346 indicates Angiotensin II/III antibody.  However, the Angiotensin II protein (http://www.novusbio.com/Serpin-A8-Angiotensinogen-Protein_NBP1-99199.html.) which you recommand to use to do the preabsorption test is Serpin A8/Angiotensinogen partial recombinant protein (38 kDa).  Which one was used to produce Angiotensin II/III antibody (NB100-62346), angiotensin II or angiotensinogen partial recombinant protein ?
   • Product NB100-62346 was made to the angiotensin II protein (aa34-41), which is a partial recombinant protein of the angiotensinogen full length protein (aa 1-485). You can use protein NBP1-99199 to perform a pre-absorption test since it is the same amino acids as the antigen used to make NB100-62346.

Bioinformatics

• Gene Symbol: AGT
• Entrez:
  • 183(Human)
• Uniprot:
  • P01019()