Angiotensin I Antibody (BGN/KA/4H) - BSA Free

Product Discontinued

Angiotensin I Antibody (BGN/KA/4H) - BSA Free Summary

• Immunogen: Synthetic peptide corresponding to amino acids 40-49 of the angiotensinogen precursor (Swiss-Prot entry P01019) conjugated to HMW carrier protein.
• Epitope: P-F-H-L-V-I-H-N-E-S(-C)
• Specificity: NB100-62344 is specific for Angiotensin I and does not react with angiotensin II, III or angiotensinogen in ELISA. Enzyme (ACE) into a vasoactive form called Angiotensin II which is responsible for increasing blood pressure. Angiotensin I is important in the Renin-Angiotensin-Aldosterone System (RAAS). RAAS is responsible for the regulation of blood pressure and water retention in the body. Angiotensin I is produced from a reaction between the renal enzyme Renin and angiotensinogen. It is converted by Angiotensin Converting Enzyme (ACE) into a vasoactive form called Angiotensin II which is responsible for increasing blood pressure.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: AGT
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:12000-1:100000
  • Western Blot
• Application Notes: Use in Western Blot reported in scientific literature (PMID:33028854).
• Control:

  Serpin A8/Angiotensinogen Overexpression Lysate

Reactivity Notes

Use in Rat reported in scientific literature (PMID:33028854).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Angiotensin I Antibody (BGN/KA/4H) - BSA Free

• alpha-1 antiproteinase, antitrypsin
• angiotensin II
• angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• angiotensinogen
• ANHU
• FLJ92595
• FLJ97926
• pre-angiotensinogen
• serine (or cysteine) proteinase inhibitor
• Serpin A8
• SERPINA8angiotensin I

Background

The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiotensin I Antibody (NB100-62344)(1)

Publication using NB100-62344

• Agier J, Brzezinska-Blaszczyk E, Witczak P et al. Ninjurin 1 dodecamer peptide containing the N-terminal adhesion motif (N-NAM) exerts proangiogenic effects in HUVECs and in the postischemic brain Cellular Immunology 2020-10-07 [PMID: 33028854] (WB, Rat, Human)

Bioinformatics

• Gene Symbol: AGT
• Entrez:
  • 183(Human)
• Uniprot:
  • P01019()