Amphiphysin/AMPH Antibody (694145) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: IHC
• Clone: 694145
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Amphiphysin/AMPH Antibody (694145) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Amphiphysin/AMPH
  Asn204-Gly300
  Accession # P49418
• Specificity: Detects human Amphiphysin/AMPH in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Amphiphysin/AMPH Antibody (694145) [Alexa Fluor® 488]

• AMPH
• AMPH1
• amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen)
• amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen)
• amphiphysin I
• Amphiphysin

Background

AMPH (Amphiphysin; also AMPH1) is a member of the Amphiphysin family, BAR superfamily of proteins.  Although its calculated MW is only 78 kDa, it runs anomalously in SDS-Page at 125-128 kDa.  It is expressed in neurons, Sertoli cells, possibly in anterior pituitary endocrine cells and apparently cardiac muscle.  It appears to form heterodimers with AMPH2, and in this configuration, promotes clathrin-associated endocytic vesicle formation.  Furthermore, and perhaps related, AMPH also interacts with N-WASP, initiating actin polymerization.  Human AMPH is 695 amino acids (aa) in length.  It contains an N-terminal BAR domain (aa 24-240) that binds lipids, a central region that binds AP2 (aa 322-340) and clathrin (aa 347-386), and a C-terminal SH3 domain (aa 622-695) that binds proline-rich ligands.  There is one known 108 kDa splice variant that shows a deletion of aa 425-466.  Over aa 204-300, human AMPH shares 98% aa identity with mouse AMPH. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P49418(Human)