Aminopeptidase PILS/ARTS1 Antibody (6H9) [Unconjugated]

Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse Aminopeptidase PILS/ARTS1 Monoclonal Antibody (Catalog # MAB2334) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Aminopeptidase PILS/ARTS1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Aminopeptidase PILS/ARTS1 at approximately 114 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse Aminopeptidase PILS/ARTS1 Monoclonal Antibody (Catalog # MAB2334). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western
• Clone: 6H9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Aminopeptidase PILS/ARTS1 Antibody (6H9) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 9-derived recombinant human ARTS1
  Accession # Q9NZ08
• Specificity: Detects human Aminopeptidase PILS/ARTS1 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse Aminopeptidase PILS/ARTS1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ERAP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aminopeptidase PILS/ARTS1 Antibody (6H9) [Unconjugated]

• Adipocyte-derived leucine aminopeptidase
• A-LAP
• A-LAPALAP
• Aminopeptidase PILS
• ARTS1
• ARTS1aminopeptidase regulator of TNFR1 shedding
• ARTS-1PILSAP
• EC 3.4.11
• EC 3.4.11.-
• EC 3.4.11.1
• endoplasmic reticulum aminopeptidase 1
• endoplasmic reticulum aminopeptidase associated with antigen processing
• ERAAP1
• ERAP1
• KIAA0525APPILS
• PILS-AP
• PILS-APERAAP
• Puromycin-insensitive leucyl-specific aminopeptidase
• Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator

Background

Aminopeptidase PILS/ARTS1 is also known as adipocyte-derived leucine aminopeptidase, type 1 tumor necrosis factor receptor shedding aminopeptidase regulator and ERAAP (the aminopeptidase associated with antigen processing in the endoplasmic reticulum). ARTS1 is widely expressed and releases an N-terminal amino acid (Leu). The amino acid sequence of mouse ARTS1 is 94%, 84%, 80 and 70% identical to that of rat, human, canine, and chicken.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aminopeptidase PILS/ARTS1 Antibody (MAB2334)(3)

Publications using MAB2334

• K Textoris-T, C Cammann, P Henklein, E Topfstedt, F Ebstein, S Henze, J Liepe, F Zhao, D Schadendor, B Dahlmann, W Uckert, A Paschen, M Mishto, U Seifert ER-aminopeptidase 1 determines the processing and presentation of an immunotherapy-relevant melanoma epitope Eur. J. Immunol., 2019-11-27;0(0):. 2019-11-27 [PMID: 31729751] (Western Blot, Human)
• Despoina Koumantou, Eilon Barnea, Adrian Martin-Esteban, Zachary Maben, Athanasios Papakyriakou, Anastasia Mpakali et al. Editing the immunopeptidome of melanoma cells using a potent inhibitor of endoplasmic reticulum aminopeptidase 1 (ERAP1) Cancer Immunology, Immunotherapy 8/1/2019 [PMID: 31222486]
• Keller M, Ebstein F, Burger E, Textoris-Taube K, Gorny X, Urban S, Zhao F, Dannenberg T, Sucker A, Keller C, Saveanu L, Kruger E, Rothkotter H, Dahlmann B, Henklein P, Voigt A, Kuckelkorn U, Paschen A, Kloetzel P, Seifert U The proteasome immunosubunits, PA28 and ER-aminopeptidase 1 protect melanoma cells from efficient MART-126-35 -specific T-cell recognition. Eur J Immunol, 2015-10-19;45(12):3257-68. 2015-10-19 [PMID: 26399368] (Western Blot, Human)

Review for Aminopeptidase PILS/ARTS1 Antibody (MAB2334) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 02/19/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22918227
• Species: Human

Bioinformatics

• Gene Symbol: ERAP1
• Uniprot:
  • Q9NZ08()