Aminopeptidase O/ONPEP Antibody (562912)

Aminopeptidase O/ONPEP was detected in immersion fixed paraffin-embedded sections of human kidney array using Human Aminopeptidase O/ONPEP Monoclonal Antibody (Catalog # MAB4647) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 562912
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Aminopeptidase O/ONPEP Antibody (562912) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Aminopeptidase O/ONPEP
  Met1-Phe819
  Accession # Q8N6M6
• Specificity: Detects human Aminopeptidase O/ONPEP in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: C9ORF3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aminopeptidase O/ONPEP Antibody (562912)

• Aminopeptidase O
• AOPEP
• APO
• AP-OFLJ55832
• C90RF3
• C9orf3
• chromosome 9 open reading frame 3
• EC 3.4.11.-
• FLJ14675
• FLJ40923
• ONPEP

Background

Aminopeptidase O (AP-O; also C9orf3) is a 93 kDa (predicted), intracellular member of the M1 metalloprotease family of enzymes. It is expressed in heart, testis and pancreas where it apparently cleaves N terminal Arg and Asn residues. Human Aminopeptidase O is 819 amino acids in length. It contains one catalytic domain (aa 167‑589) with a Zn-binding site (His479-His483) and a C terminal ARM region (aa 706-819). At least three additional isoforms exist. One shows a deletion of aa 455‑553; a second has an 11 aa substitution for the C terminal 265 amino acids; and a third shows both a deletion of aa 455‑553 and an 11 aa substitution for the C-terminal 46 amino acids. Full length human Aminopeptidase O is 76% aa identical to mouse Aminopeptidase O.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C9ORF3
• Uniprot:
  • Q8N6M6()