Aminopeptidase A/ENPEP Antibody

• Reactivity: Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Aminopeptidase A/ENPEP Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Aminopeptidase A/ENPEP
  Arg41-Pro945
  Accession # P16406
• Specificity: Detects mouse Aminopeptidase A/ENPEP in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human ENPEP is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ENPEP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aminopeptidase A/ENPEP Antibody

• Aminopeptidase A
• APA
• AP-A
• CD249 antigen
• CD249
• Differentiation antigen gp160
• EAP
• EC 3.4.11
• EC 3.4.11.7
• ENPEP
• glutamyl aminopeptidase (aminopeptidase A)
• glutamyl aminopeptidase
• gp160

Background

The mouse ENPEP gene encodes Aminopeptidase A (APA), which is also known as glutamyl aminopeptidase (EAP), aspartate aminopeptidase, angiotensinase A, Ca²⁺-activated glutamate aminopeptidase, membrane aminopeptidase II and the BP-1/6C3 antigen (2). The various names reflect its substrate specificity (prefer Glu or Asp and angiotensin II as one of the physiological substrates), Ca²⁺-dependence, cellular location (type II membrane protein) and immunological reactivity to antibody BP-1/6C3. The deduced amino acid sequence of mouse ENPEP consists of a short cytoplasmic tail (residues 1 to 18), a transmembrane region (residue 19 to 39), and a long ectodomain (residues 40 to 945) (1). In addition to the N-terminal zinc metalloprotease domain, the ectodomain also contains the C‑terminal region, which functions as an intramolecular chaperone required for the correct folding, cell surface expression and activity (3). The purified rmENPEP consists of the ectodomain and is active in the assay described above.

1. Wu, Q. et al. (1990) Proc. Natl. Acad. Sci. USA 87:993.
2. Wang, J. and M.D. Cooper (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 299, Academic Press, San Diego.
3. Rozenfeld, R. et al. (2004) J. Biol. Chem. 279:43285.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aminopeptidase A/ENPEP Antibody (AF2809)(1)

Publication using AF2809

• PS Caceres, D Gravotta, PJ Zager, N Dephoure, E Rodriguez- Quantitative proteomics of MDCK cells identify unrecognized roles of clathrin adaptor AP-1 in polarized distribution of surface proteins Proc. Natl. Acad. Sci. U.S.A., 2019-05-29;116(24):11796-11805. 2019-05-29 [PMID: 31142645] (Western Blot, Canine)

Bioinformatics

• Gene Symbol: ENPEP
• Uniprot:
  • P16406()