AMID Antibody - BSA Free

Western Blot: AMID Antibody - BSA Free [NBP3-18132] - WB Validation in Rat Tissues. Loading: 15 ug of lysate. Antibodies: AMID NBP3-18132, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.

Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18132] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-AMID antibody (NBP3-18132) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Western Blot: AMID Antibody - BSA Free [NBP3-18132] - WB Validation in Human Mouse and Rat Cell Lines. Loading: 15 ug of lysate. Antibodies: AMID NBP3-18132, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.

Western Blot: AMID Antibody - BSA Free [NBP3-18132] - WB Validation in Mouse Tissues. Loading: 15 ug of lysate. Antibodies: AMID NBP3-18132, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10,000 dilution.

Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18132] - Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-AMID antibody (NBP3-18132) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry-Paraffin: AMID Antibody - BSA Free [NBP3-18132] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-AMID antibody (NBP3-18132) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence: AMID Antibody - BSA Free [NBP3-18132] -Validation of AIFM2 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling AIFM2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

• Reactivity: Hu, Mu, Rt, Bv, Pm, Eq
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

AMID Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit AMID Antibody - BSA Free (NBP3-18132) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 12 amino acids near the carboxyl terminus of human AMID. The immunogen is located within the last 50 amino acids of AMID.
• Predicted Species: Bovine (92%), Chimpanzee (100%), Equine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AIFM2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 2 ug/mL
• Control Peptide:

  AMID Protein (NBP3-18132PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for AMID Antibody - BSA Free

• AMID
• apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death
• apoptosis-inducing factor 2
• Apoptosis-inducing factor homologous mitochondrion-associated inducer of death
• apoptosis-inducing factor, mitochondrion-associated, 2
• Apoptosis-inducing factor-like mitochondrion-associated inducer of death
• DKFZp686L1298
• FLJ14497
• p53-responsive gene 3 protein
• PRG3apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer ofdeath
• RP11-367H5.2

Background

AMID is encoded by this gene has significant homology to NADH oxidoreductases and the apoptosis-inducing factor PDCD8/AIF. Overexpression of this gene has been shown to induce apoptosis. The expression of this gene is found to be induced by tumor suppressor protein p53 in colon caner cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AIFM2