AMBRA1 Antibody

Product Discontinued

AMBRA1 Antibody Summary

• Immunogen: This antibody was made against a protein fragment from the C Terminus Region of AMBRA1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AMBRA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Immunoprecipitation use reported in customer review. Use in Rat reported in customer review. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27123694).

Reactivity Notes

Human. Cross reactivity with rat reported in customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs. These products are available in limited quantities. Please enquire about the availability of this product before purchasing.

Alternate Names for AMBRA1 Antibody

• activating molecule in BECN1-regulated autophagy protein 1
• autophagy/beclin-1 regulator 1
• DCAF3
• DDB1 and CUL4 associated factor 3
• FLJ20294
• KIAA1736activating molecule in beclin-1-regulated autophagy
• MGC33725
• WD repeat domain 94
• WDR94

Background

AMBRA1 regulates autophagy and development of the nervous system. It is a large, previously unknown protein bearing a WD40 domain at its amino terminus, regulates autophagy and has a crucial role in embryogenesis. AMBRA1 is a positive regulator of the Becn1-dependent programme of autophagy, as revealed by its overexpression and by RNA interference experiments invitro. Notably, AMBRA1 functional deficiency in mouse embryos leads to severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AMBRA1 Antibody (26190002)(12)

Publications using 26190002

• Sanchez-Martin P, Lahuerta M, Viana R et al. Regulation of the autophagic PI3KC3 complex by laforin/malin E3-ubiquitin ligase, two proteins involved in Lafora disease Biochim Biophys Acta Mol Cell Res. 2019-11-21 [PMID: 31758957] (WB, Human)
• Di Rienzo M, Antonioli M, Fusco C et al. Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains Sci Adv 2019-05-01 [PMID: 31123703] (WB, KD, Human, Mouse)
• Nardella G, Visci G, Guarnieri V et al. A single-center study on 140 patients with cerebral cavernous malformations: 28 new pathogenic variants and functional characterization of a PDCD10 large deletion. Hum. Mutat. 2018-08-30 [PMID: 30161288] (WB, Human)
• Li H, Ham A, Ma TC et al. Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations Autophagy 2019-01-01 [PMID: 30160596] (WB, Human)
• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Strappazzon F, Di Rita A, Cianfanelli V et al. Prosurvival AMBRA1 turns into a proapoptotic BH3-like protein during mitochondrial apoptosis. Autophagy. 2016-04-28 [PMID: 27123694] (ICC/IF, WB, Human)
• Chauhan S, Mandell MA, Deretic V. IRGM Governs the Core Autophagy Machinery to Conduct Antimicrobial Defense Mol. Cell 2015-04-15 [PMID: 25891078] (WB, Human)
• Wakabayashi K, Mori F, Kakita A et al. Analysis of microRNA from archived formalin-fixed paraffin-embedded specimens of amyotrophic lateral sclerosis. Acta Neuropathol Commun. 2014-12-14 [PMID: 25497327] (IHC-P, Human)
• Cianfanelli V, Fuoco C, Lorente M et al. AMBRA1 links autophagy to cell proliferation and tumorigenesis by promoting c-Myc dephosphorylation and degradation. Nat Cell Biol 2015-01-01 [PMID: 25438055] (WB)
• Strappazzon F, Nazio F, Corrado M et al. AMBRA1 is able to induce mitophagy via LC3 binding, regardless of PARKIN and p62/SQSTM1. Cell Death Differ. 2014-09-12 [PMID: 25215947]
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.
• Nazio F, Strappazzon F, Antonioli M. mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6 Nat Cell Biol 2013-04-01 [PMID: 23524951] (WB, Human)

Review for AMBRA1 Antibody (26190002) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer IP 05/15/2012

Summary

• Application: Immunoprecipitation

Bioinformatics

• Gene Symbol: AMBRA1
• Entrez:
  • 55626(Human)
• Uniprot:
  • Q9C0C7(Human)