AlphaB Crystallin/CRYAB Antibody

Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Human A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 and T98G cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: A431 cell lysates. Lane 3: HCT116 cell lysates. Lane 4: HeLa cell lysates. Lane 5: HepG2 cell lysates. Lane 6: HEK293 cell lysates. Lane 7: HUVEC cell lysates. Lane 8: Jurkat cell lysates. Lane 9: MCF7 cell lysates. Lane 10: PC3 cell lysates. Lane 11: T98G cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.

Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - analysis using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody. Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 20x. Heat Shocked at 42C for 1h.

Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.

Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 100x. Heat Shocked at 42C for 1h.

• Reactivity: Hu, Mu, Rt, Bv, Ch
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

AlphaB Crystallin/CRYAB Antibody Summary

• Immunogen: Synthetic peptide corresponding to human alpha B crystallin conjugated to KLH
• Localization: Cytoplasm, Nucleus
• Specificity: Detects approx 22kDa. Does not cross-react with alpha A-crystallin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRYAB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:5000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: A 1:5000 dilution of Alpha B Crystallin Antibody was sufficient for detection of alpha B crystallin in 20 ug of HeLa cell lysate by ECL immunoblot analysis.
• Control:

  AlphaB Crystallin/CRYAB Overexpression Lysate

Reactivity Notes

Based on homology, It is predicted to detect Mouse, Rat, Bovine and Chicken

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS pH 7.4, 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for AlphaB Crystallin/CRYAB Antibody

• alpha B crystallin
• alpha(B)-crystallin
• AlphaB Crystallin
• alpha-crystallin B chain
• CRYA2
• CRYA2alpha crystallin B chain
• CRYAB
• crystallin, alpha B
• CTPP2
• Heat shock protein beta-5
• heat-shock 20 kD like-protein
• HSPB5
• Renal carcinoma antigen NY-REN-27
• Rosenthal fiber component

Background

Lens proteins consist almost entirely of Crystallins (about 95%). Crystallins are also found in vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha Crystallin is the largest of the crystallins and is composed of 2 primary gene products; alpha A and alpha B. There are at least 5 different proteins comprising the beta Crystallins. The gamma Crystallins are monomeric, but there are at least 5 gamma Crystallins identified in bovine and rat lens. Alpha Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha Crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha B Crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AlphaB Crystallin/CRYAB Antibody (NB120-13497)(1)

Publication using NB120-13497

• Gelman BB, Nguyen TP Synaptic proteins linked to HIV-1 infection and immunoproteasome induction: proteomic analysis of human synaptosomes. J Neuroimmune Pharmacol. 2010 Mar 1 [PMID: 19693676] (WB, Human)

Bioinformatics

• Gene Symbol: CRYAB
• Entrez:
  • 1410(Human)
  • 12955(Mouse)
  • 25420(Rat)
• Uniprot:
  • NP_001876.1(Human)