AlphaA Crystallin/CRYAA Antibody

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Western Blot: AlphaA Crystallin/CRYAA Antibody [NB120-5595] - Analysis of alpha-A crystallin from bovine lens extract.

Product Details

Summary
Reactivity Rt, BvSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

AlphaA Crystallin/CRYAA Antibody Summary

Immunogen
Purified alpha-A Crystallin from bovine lens.
Localization
Cytoplasmic
Specificity
This does not detect the unphosphorylated form of the protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CRYAA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/ml
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
Application Notes
WB: Detects an approx. 20 kDa representing alpha-A crystallin from bovine lens samples. IHC: Staining of alpha-A crystallin in rat thymus samples results in staining of epithelial reticular cells.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 30 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute to desired concentration.

Alternate Names for AlphaA Crystallin/CRYAA Antibody

  • AlphaA Crystallin
  • CRYA1
  • CRYA1crystallin, alpha-1
  • CRYAA
  • crystallin, alpha A
  • Heat shock protein beta-4
  • HSPB4
  • HSPB4alpha-crystallin A chain
  • human alphaA-crystallin (CRYA1), 10HspB4

Background

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CRYAA
Entrez