AlphaA Crystallin/CRYAA Antibody (1H3.B8) - BSA Free

Western Blot: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875] - Western Blot analysis of Bovine tissue lysate showing detection of AlphaA Crystallin/CRYAA protein using Mouse Anti-AlphaA Crystallin/CRYAA Monoclonal Antibody, Clone 1H3.B8 (NBP2-12875). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-AlphaA Crystallin/CRYAA Monoclonal Antibody (NBP2-12875) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. This blot shows absolute specificity as Left: AlphaA Crystallin/CRYAA, Right: Alpha B Crystallin.

Immunocytochemistry/Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-AlphaA Crystallin/CRYAA Monoclonal Antibody, Clone 1H3.B8 (NBP2-12875). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-AlphaA Crystallin/CRYAA Monoclonal Antibody (NBP2-12875) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) AlphaA Crystallin/CRYAA Antibody. (D) Composite.

Immunocytochemistry/Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha A Crystallin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF
• Clone: 1H3.B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

AlphaA Crystallin/CRYAA Antibody (1H3.B8) - BSA Free Summary

• Immunogen: Native Alpha Crystallin
• Localization: Cytoplasm , Nucleus
• Specificity: Detects approx 20kDa. Does not cross-react with aB-crystallin, Beta L-crystallin, BH- crystallin, gamma-crystallin, HSP25, HSP27 or HSP47 proteins.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRYAA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Application Notes: 0.5 ug/ml was sufficient for detection of 100 ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.Alpha A Crystallin Antibody.Use in Immunoblotting reported in scientific literature (PMID 28546921)..

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for AlphaA Crystallin/CRYAA Antibody (1H3.B8) - BSA Free

• AlphaA Crystallin
• CRYA1
• CRYA1crystallin, alpha-1
• CRYAA
• crystallin, alpha A
• Heat shock protein beta-4
• HSPB4
• HSPB4alpha-crystallin A chain
• human alphaA-crystallin (CRYA1), 10HspB4

Background

0.5ug/ml was sufficient for detection of 100ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AlphaA Crystallin/CRYAA Antibody (NBP2-12875)(1)

Publication using NBP2-12875

• Cui XK, Zhu KK, Zhou Z et al. A novel frameshift mutation in CX46 associated with hereditary dominant cataracts in a Chinese family. Int J Ophthalmol 2017-05-26 [PMID: 28546921] (IB, Human)

Bioinformatics

• Gene Symbol: CRYAA
• Uniprot:
  • NP_000385.1(Human)