AlphaA Crystallin/CRYAA Antibody (1H3.B8)

Images

 
Western Blot: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875] - analysis of Bovine tissue lysate showing detection of Alpha A Crystallin protein using Mouse Anti-Alpha A Crystallin Monoclonal Antibody, Clone ...read more
Immunocytochemistry/ Immunofluorescence: AlphaA Crystallin/CRYAA Antibody (1H3.B8) [NBP2-12875] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IB, ICC/IF
Clone
1H3.B8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

AlphaA Crystallin/CRYAA Antibody (1H3.B8) Summary

Immunogen
Native Alpha Crystallin
Localization
Cytoplasm, Nucleus
Specificity
Detects approx 20kDa. Does not cross-react with aB-crystallin, Beta L-crystallin, BH- crystallin, gamma-crystallin, HSP25, HSP27 or HSP47 proteins.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CRYAA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • ELISA 1:100-1:2000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
Application Notes
0.5 ug/ml was sufficient for detection of 100 ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.Alpha A Crystallin Antibody.Use in Immunoblotting reported in scientific literature (PMID 28546921)..
Publications
Read Publication using
NBP2-12875 in the following applications:

  • IB
    1 publication

Reactivity Notes

Based on homology, It's predicted to detect Mouse, Rat and Bovine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for AlphaA Crystallin/CRYAA Antibody (1H3.B8)

  • AlphaA Crystallin
  • CRYA1
  • CRYA1crystallin, alpha-1
  • CRYAA
  • crystallin, alpha A
  • Heat shock protein beta-4
  • HSPB4
  • HSPB4alpha-crystallin A chain
  • human alphaA-crystallin (CRYA1), 10HspB4

Background

0.5ug/ml was sufficient for detection of 100ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AlphaA Crystallin/CRYAA Antibody (NBP2-12875)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IB.


Filter By Application
IB
(1)
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Filter By Species
Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Diseases for AlphaA Crystallin/CRYAA Antibody (NBP2-12875)

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Bioinformatics

Gene Symbol CRYAA
Entrez
Uniprot