alpha-Taxilin Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human a-Taxilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5575) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for a-Taxilin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

a-Taxilin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human a-Taxilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5575) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

alpha-Taxilin Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human alpha -Taxilin
  Met1-Lys162
  Accession # P40222
• Specificity: Detects human alpha -Taxilin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TXLNA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Taxilin Antibody [Unconjugated]

• alphaTaxilin
• alpha-Taxilin
• DKFZp451J0118
• IL14
• IL-14
• interleukin 14
• MGC118870
• MGC118871
• RP4-622L5.4
• taxilin alpha
• TXLN
• TXLNA

Background

alpha ‑Taxilin (TXLN‑ alpha ; also p75 [in rat] and IL‑14 alpha ) is the founding member of the taxilin family of proteins. Although its predicted MW is 62 kDa, it runs anomalously at 74‑76 kDa in SDS‑PAGE. alpha ‑Taxilin is ubiquitously expressed and participates in intracellular vesicle trafficking. In particular, alpha ‑Taxilin interacts with syntaxin family members that localize to the plasma membrane, providing for SNARE complex formation. alpha ‑Taxilin also regulates the placement of growing protein chains. Human alpha ‑Taxilin is 546 amino acids (aa) in length. Its defining characteristic is a large coiled‑coil region between aa 186‑491. When an alternate start site at Met176 is used, a secreted cytokine is generated termed IL‑14 alpha . Over aa 1‑162, human alpha ‑Taxilin shares 81% aa identity with mouse alpha ‑Taxilin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TXLNA
• Uniprot:
  • P40222()