alpha-Synuclein Antibody (2A7)

Images

 
Western Blot: Synuclein-alpha Antibody (2A7) [NBP1-05194] - Blots of NBP1-05194 on recombinant alpha-synuclein (left lane) and crude extract of mouse brain (right lane, courtesy Rogan Tinsley, Howard Florey Institute, ...read more
Immunocytochemistry/ Immunofluorescence: Synuclein-alpha Antibody (2A7) [NBP1-05194] - Mixed neuron-glial cultures stained with NBP1-05194, our monoclonal antibody to alpha-synuclein (red) and chicken polylclonal ...read more
Simple Western: Synuclein-alpha Antibody (2A7) [NBP1-05194] - Simple Western lane view shows a specific band for alpha-Synuclein in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clone
2A7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

alpha-Synuclein Antibody (2A7) Summary

Immunogen
Human alpha-Synuclein [UniProt# P37840]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SNCA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:2000
  • Immunocytochemistry/Immunofluorescence 1:1000
Application Notes
This alpha-Synuclein antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
14.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for alpha-Synuclein Antibody (2A7)

  • alpha-Synuclein
  • Lewy body) 4
  • MGC110988
  • NACP
  • non A-beta component of AD amyloid
  • Non-A beta component of AD amyloid
  • Non-A4 component of amyloid precursor
  • non-A4 component of amyloid
  • PARK1
  • PARK4
  • PD1
  • SNCA
  • synuclein, alpha (non A4 component of amyloid precursor)
  • Synuclein-alpha

Background

alpha-Synuclein (SNCA, NACP or PARK-1) is a neuronal protein normally localized to presynaptic terminals and has been involved in processes such as dopamine biosynthesis, synaptic plasticity, and vesicle dynamics. SNCA-VAMP2 direct binding promotes SNARE complex assembly through a nonclassical chaperone activity and regulation of SNARE complex's assembly/disassembly is essential for efficient neurotransmission. CSP-alpha, a presynaptic protein also promotes SNARE complex assembly via formation of chaperone complex with heat shock cognate 70 (Hsc70) and small glutamine-rich protein SGT (CSP-alpha-Hsc70-SGT complex). Interestingly, the CSP-alpha-knockout mice depicted rapidly progressive neurodegeneration followed by premature death which was counteracted by transgenic expression of SNCA. Overexpressed SNCA indirectly inhibited SNARE-mediated exocytosis by sequestering arachidonic acid, which upregulates syntaxin and enhances its engagement with SNARE complex. Tight regulation of subcellular level and distribution of SNCA is indispensable for the intrinsic functions of neuronal cells and genetic alterations of SNCA resulting in aberrant polymerization into fibrils are associated with several neurodegenerative diseases (synucleinopathies) including Parkinson disease type -1 (PARK1) - 4 (PARK4) as well as dementia Lewy body (DLB).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha-Synuclein Antibody (NBP1-05194). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for an alpha-Synuclein antibody with an epitope located in the first half (N-terminus) of the protein - preferably a monoclonal antibody. Can you help me with that?
    • Please take a look at NB110-57475. It has been validated for human, rat and mouse and the applications ICC and WB and the epitope it detects is in the N terminal.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SNCA
Entrez
Uniprot