| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92438-0.02ml | |||
HAF018). A specific band was detected for a-Sarcoglycan at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human skeletal muscle tissue and human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human a-Sarcoglycan Monoclonal Antibody (Catalog # MAB7445) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Sarcoglycan at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7445-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90306-25ul | |||
![Western Blot: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Western blot analysis of alpha-Sarcoglycan on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse heart tissue lysate Lane 2: human skeletal muscle tissue lysate](http://images.novusbio.com/fullsize/alpha-Sarcoglycan-Antibody-JA51-81-Western-Blot-NBP2-67150-img0006.jpg "Western Blot: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Western blot analysis of alpha-Sarcoglycan on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse heart tissue lysate Lane 2: human skeletal muscle tissue lysate")
![Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Sarcoglycan-Antibody-JA51-81-Immunohistochemistry-Frozen-NBP2-67150-img0005.jpg)
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Sarcoglycan-Antibody-JA51-81-Immunohistochemistry-Paraffin-NBP2-67150-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/alpha-Sarcoglycan-Antibody-JA51-81-Immunohistochemistry-Paraffin-NBP2-67150-img0002.jpg)
![Western Blot: alpha-Sarcoglycan Antibody [NBP2-92438] - Analysis of extracts of various cell lines, using alpha-Sarcoglycan at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.](http://images.novusbio.com/fullsize/alpha-Sarcoglycan-Antibody-Western-Blot-NBP2-92438-img0001.jpg "Western Blot: alpha-Sarcoglycan Antibody [NBP2-92438] - Analysis of extracts of various cell lines, using alpha-Sarcoglycan at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: alpha-Sarcoglycan Antibody [NBP2-92438] - Analysis of C6 cells using alpha Sarcoglycan (alpha Sarcoglycan (SGCA)) Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/alpha-Sarcoglycan-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92438-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha-Sarcoglycan Antibody - Azide and BSA Free [NBP2-92438] - Immunofluorescence analysis of U2OS cells using alpha Sarcoglycan Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92438_rabbit-polyclonal-alpha-sarcoglycan-antibody-42202514264494.jpg%3Ft=1709198919)
![Immunocytochemistry/ Immunofluorescence: alpha-Sarcoglycan Antibody - Azide and BSA Free [NBP2-92438] - Immunofluorescence analysis of L929 cells using alpha Sarcoglycan Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92438_rabbit-polyclonal-alpha-sarcoglycan-antibody-42202514251830.jpg%3Ft=1709198919)
![Western Blot: alpha-Sarcoglycan Antibody (3W3Q10) [NBP3-15896] - Western blot analysis of extracts of various cell lines, using alpha-Sarcoglycan (SGCA) Rabbit mAb (NBP3-15896) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/alpha-Sarcoglycan-Antibody-3W3Q10-Western-Blot-NBP3-15896-img0005.jpg "Western Blot: alpha-Sarcoglycan Antibody (3W3Q10) [NBP3-15896] - Western blot analysis of extracts of various cell lines, using alpha-Sarcoglycan (SGCA) Rabbit mAb (NBP3-15896) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (3W3Q10) [NBP3-15896] - Immunohistochemistry of paraffin-embedded mouse heart using alpha-Sarcoglycan (SGCA) Rabbit mAb (NBP3-15896) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/alpha-Sarcoglycan-Antibody-3W3Q10-Immunohistochemistry-Paraffin-NBP3-15896-img0002.jpg)
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (3W3Q10) [NBP3-15896] - Immunohistochemistry of paraffin-embedded rat heart using alpha-Sarcoglycan (SGCA) Rabbit mAb (NBP3-15896) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/alpha-Sarcoglycan-Antibody-3W3Q10-Immunohistochemistry-Paraffin-NBP3-15896-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha-Sarcoglycan Antibody (3W3Q10) [alpha-Sarcoglycan] - Confocal imaging of paraffin-embedded Rat heart using alpha-Sarcoglycan Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15896_rabbit-alpha-sarcoglycan-mab-3w3q10-5220251718941.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha-Sarcoglycan Antibody (3W3Q10) [alpha-Sarcoglycan] - Confocal imaging of paraffin-embedded Mouse heart using alpha-Sarcoglycan Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15896_rabbit-alpha-sarcoglycan-mab-3w3q10-52202517223920.jpg)
![Western Blot: alpha-Sarcoglycan Antibody [NBP3-12370] - 1:750 antibody in DiluObuffer.](http://images.novusbio.com/fullsize/alpha-Sarcoglycan-Antibody-Western-Blot-NBP3-12370-img0002.jpg "Western Blot: alpha-Sarcoglycan Antibody [NBP3-12370] - 1:750 antibody in DiluObuffer.")
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody [NBP3-12370] - Mouse large intestine. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/alpha-Sarcoglycan-Antibody-Immunohistochemistry-Paraffin-NBP3-12370-img0001.jpg)
![Western Blot: alpha-Sarcoglycan Antibody [NBP1-90306] - Analysis in control (vector only transfected HEK293T lysate) and SGCA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/alpha-Sarcoglycan-Antibody-Western-Blot-NBP1-90306-img0005.jpg "Western Blot: alpha-Sarcoglycan Antibody [NBP1-90306] - Analysis in control (vector only transfected HEK293T lysate) and SGCA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody [NBP1-90306] - Staining of human heart muscle shows distinct positivity of intercalated discs in myocytes.](http://images.novusbio.com/fullsize/alpha-Sarcoglycan-Antibody-Immunohistochemistry-Paraffin-NBP1-90306-img0001.jpg)
![Western Blot: alpha-Sarcoglycan Antibody (3C4) [H00006442-M01] - Analysis of SGCA expression in transfected 293T cell line by SGCA monoclonal antibody (M01), clone 3C4.Lane 1: SGCA transfected lysate(42.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/alpha-Sarcoglycan-Antibody-3C4-Western-Blot-H00006442-M01-img0004.jpg "Western Blot: alpha-Sarcoglycan Antibody (3C4) [H00006442-M01] - Analysis of SGCA expression in transfected 293T cell line by SGCA monoclonal antibody (M01), clone 3C4.Lane 1: SGCA transfected lysate(42.9 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: alpha-Sarcoglycan Antibody (3C4) [H00006442-M01] - Detection limit for recombinant GST tagged SGCA is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/alpha-Sarcoglycan-Antibody-3C4-Sandwich-ELISA-H00006442-M01-img0003.jpg)
