alpha-N-acetylgalactosaminidase/NAGA Overexpression Lysate (Native)

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Western Blot: NAGA Overexpression Lysate (Adult Normal) [NBL1-13462] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NAGA.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA

Order Details

alpha-N-acetylgalactosaminidase/NAGA Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200707 and based on accession number NM_000262. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens N-acetylgalactosaminidase, alpha- (NAGA), mRNA.
Gene
NAGA

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
44.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBL1-13462 in the following application:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for alpha-N-acetylgalactosaminidase/NAGA

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for alpha-N-acetylgalactosaminidase/NAGA Overexpression Lysate (Native)

  • alpha-N- (alpha-galactosidase B)
  • alphaNacetylgalactosaminidase
  • alpha-N-acetylgalactosaminidase
  • EC 3.2.1
  • EC 3.2.1.49
  • GALB
  • N-acetylgalactosaminidase, alpha-
  • NAGA

Background

NAGA encodes the lysosomal enzyme alpha-N-acetylgalactosaminidase, which cleaves alpha-N-acetylgalactosaminyl moieties from glycoconjugates. Mutations in NAGA have been identified as the cause of Schindler disease types I and II (type II also known as Kanzaki disease). [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for alpha-N-acetylgalactosaminidase/NAGA Lysate (NBL1-13462) (0)

There are no publications for alpha-N-acetylgalactosaminidase/NAGA Lysate (NBL1-13462).
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Review for alpha-N-acetylgalactosaminidase/NAGA Lysate (NBL1-13462) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 application: ELISA.

Reviews using NBL1-13462:
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(1)
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reviewed by:
Patricia Maliff
ELISA 06/02/2010
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Summary

ApplicationELISA
Lot08276 WULz

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Patricia Maliff
06/02/2010
Application: ELISA
Species:

Bioinformatics

Gene Symbol NAGA
Entrez