alpha-Methylacyl-CoA Racemase/AMACR Antibody [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

alpha-Methylacyl-CoA Racemase/AMACR Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human α‑Methylacyl‑CoA Racemase/AMACR
  Met1-Leu382
  Accession # Q9UHK6
• Specificity: Detects human alpha ‑Methylacyl‑CoA Racemase/AMACR in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Methylacyl-CoA Racemase/AMACR Antibody [Alexa Fluor® 700]

• 2-methylacyl-CoA racemase
• alpha-Methylacyl-CoA Racemase
• alphaMethylacylCoARacemase
• AMACR
• AMACRD
• CBAS4
• EC 5.1.99.4
• RACE
• RM

Background

AMACR (Alpha-MethylAcyl-CoA Racemase; also 2-methylacyl racemase) is a 43-46 kDa member of the CaiB/BaiF CoA-transferase family of enzymes. It is widely expressed, being found in fibroblasts, hepatocytes, plus tumorigenic prostatic and colonic epithelium. Within these cells, it is localized to peroxisomes (organelles that participate in the breakdown fatty acids into 2-carbon blocks) and occasionally mitochondria, and appears to racemize 2-methyl-branched fatty acids. This ability is necessary for the degradation of branched fatty acids such as C19 dietary pristanic acid. Pristanic acid occurs in both an S- and R-methylated stereoisomer, but can only be initially degraded in the S- isomeric form. AMACR converts the R- to the S-isoform, initiating fatty acid processing. Human AMACR(-IA) is 382 amino acids (aa) in length. It contains an N-terminal mitochondrial targeting sequence (aa 22-85) that overlaps the enzymatic region (aa 53-231), and a C-terminal peroxisomal targeting motif (aa 379-382). There are multiple potential splice variants. Over aa 132-382, there are three aa substitutions, one that is 66 aa in length (AMACR‑IB), a second that is 147 aa in length (AMACR-IIB), and a third that is 98 aa in length. Over aa 249-382, there are two aa substitutions, one that is 13 aa in length (AMACR‑IIAs), and another that is 41 aa in length (AMACR-IIA). There is also a sixth potential splice variant that shows a 16 aa substitution for aa 378-382. Full-length human AMACR(-IA) shares 77% aa sequence identity with mouse AMACR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.