Alpha-mannosidase II Antibody (1G9) - Azide and BSA Free

Western Blot: Alpha-mannosidase II Antibody (1G9) [H00004124-M01] - Detection against Immunogen (36.74 KDa) .

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 1G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Alpha-mannosidase II Antibody (1G9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MAN2A1 (NP_002363, 1045 a.a. ~ 1144 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DIHLVNLRTIQSKVGNGHSNEAALILHRKGFDCRFSSKGTGLFCSTTQGKILVQKLLNKFIVESLTPSSLSLMHSPPGTQNISEINLSPMEISTFRIQLR
• Localization: Golgi Apparatus
• Specificity: MAN2A1 - mannosidase, alpha, class 2A, member 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAN2A1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Alpha-mannosidase II Antibody (1G9) - Azide and BSA Free

• alpha-mannosidase 2
• AMan II
• EC 3.2.1
• EC 3.2.1.114
• Golgi alpha-mannosidase II
• golgi integral membrane protein 7
• GOLIM7
• Man II
• MANA2MANII
• Mannosidase alpha class 2A member 1
• mannosidase, alpha type II
• mannosidase, alpha, class 2A, member 1
• Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

Background

This gene encodes a protein which is a member of family 38 of the glycosyl hydrolases. The protein is located in the Golgi and catalyzes the final hydrolytic step in the asparagine-linked oligosaccharide (N-glycan) maturation pathway. Mutations in the mouse homolog of this gene have been shown to cause a systemic autoimmune disease similar to human systemic lupus erythematosus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Alpha-mannosidase II Antibody (H00004124-M01)(2)

Publications using H00004124-M01

• Denis N, Palmer-Smith H, Elisma F et al. Quantitative Proteomic Analysis of PCSK9 Gain of Function in Human Hepatic HuH7 Cells. J Proteome Res. 2011-03-10 [PMID: 21332221]
• Farina AR, Tacconelli A, Cappabianca L et al. The Alternative TrkAIII Splice Variant Targets the Centrosome Promotes Genetic Instability. Mol Cell Biol;29(17):4812-4830. 2009-01-01 [PMID: 19564412]

Bioinformatics

• Gene Symbol: MAN2A1
• Entrez:
  • 4124(Human)
• OMIM:
  • 154582(Human)
• Uniprot:
  • NP_002363(Human)