Human alpha-Fetoprotein/AFP ELISA Kit (Colorimetric)

ELISA: alpha-Fetoprotein/AFP ELISA Kit [HRP] [KA0202] - Standard curve

Product Discontinued

Human alpha-Fetoprotein/AFP ELISA Kit (Colorimetric) Summary

• Description: AFP (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of alpha-fetoprotein.
• Standard Curve Range: 5 to 300 ng/mL
• Sensitivity: 2 ng/mL
• Assay Type: Sandwich ELISA
• Sample Volume: 20 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: AFP

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification
• Application Notes: Useful for quantification.

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human alpha-Fetoprotein/AFP ELISA Kit (Colorimetric)

• AFP
• Alpha-1-fetoprotein
• alpha-fetoglobulin
• alpha-Fetoprotein
• DSCAM2
• FETA
• HP
• HPAFP

Background

Alpha 1 Fetoprotein encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for alpha-Fetoprotein/AFP ELISA Kit (KA0202)(6)

Publications using KA0202

• Danoy M, Bernier ML, Kimura K et al. Optimized protocol for the hepatic differentiation of iPSCs in a fluidic microenvironment. Biotechnol Bioeng. 2019-03-29 [PMID: 30883676]
• Zhou Y, Shou F, Zhang H, You Q Adenovirus-delivered wwox inhibited lung cancer growth in vivo in a mouse model. Cancer Gene Ther 2016-01-01 [PMID: 26516139]
• Toda M, Arima Y, Takiguchi H, Iwata H. Surface plasmon field-enhanced fluorescence spectroscopy apparatus with a convergent optical system for point-of-care testing. Anal Biochem. 2014-09-04 [PMID: 25197029]
• Ong HT, Federspiel MJ, Guo CM et al. Systemically delivered measles virus-infected mesenchymal stem cells can evade host immunity to inhibit liver cancer growth. J Hepatol. 2013-07-15 [PMID: 23867315]
• Giannetto M, Elviri L, Careri M et al. A voltammetric immunosensor based on nanobiocomposite materials for the determination of alpha-fetoprotein in serum. Biosens Bioelectron. 2010-09-29 [PMID: 20947323]
• Wu S, Chen J, Tian Y et al. Biofunctionalized dendritic polyaniline nanofiber for in situ amplified glucometer-based enzyme immunoassay of tumor marker. Anal. Methods 2016-03-03

FAQs for alpha-Fetoprotein/AFP ELISA Kit (KA0202). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: AFP