alpha-Fetoprotein/AFP Antibody

Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody [NB100-1611] - Formalin fixed paraffin embedded human liver hepatocellular carcinoma stained with alpha-1 Fetoprotein antibody (NB100-1611).

Product Discontinued

alpha-Fetoprotein/AFP Antibody Summary

• Immunogen: Alpha-fetoprotein purified from human cord serum.
• Localization: Secreted, Cytoplasmic
• Specificity: This antibody reacts with ALPHA-1-fetoprotein. A positive staining in the hepatocytes of fetal tissue was observed. This antibody does not react with any other cell or tissue types.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AFP
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:150
• Application Notes: IHC-P: Use ABC method. Positive staining in the hepatocytes of fetal tissue was observed. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23874411)
• Control:

  alpha-Fetoprotein/AFP Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: None, containing less than 0.1% sodium azide
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for alpha-Fetoprotein/AFP Antibody

• AFP
• Alpha-1-fetoprotein
• Alpha-fetoglobulin
• alpha-Fetoprotein
• DSCAM2
• FETA
• HP
• HPAFP

Background

AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha-Fetoprotein/AFP Antibody (NB100-1611)(1)

Publication using NB100-1611

• Krueger WH, Tanasijevic B, Barber V et al. Cholesterol-Secreting and Statin-Responsive Hepatocytes from Human ES and iPS Cells to Model Hepatic Involvement in Cardiovascular Health. PLoS One 2013-07-11 [PMID: 23874411] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: AFP
• Entrez:
  • 174(Human)