alpha-Fetoprotein/AFP Antibody [Alexa Fluor® 488]

• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

alpha-Fetoprotein/AFP Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse α‑Fetoprotein/AFP
  Lys19-Val605
  Accession # P02772
• Specificity: Detects mouse alpha ‑Fetoprotein/AFP in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with human alpha ‑Fetoprotein is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

AFP ( alpha -Fetoprotein) is a 69‑73 kDa member of the ALB/AFP/VDB family of proteins. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature mouse AFP is 587 amino acids (aa) in length. It contains three albumin domains (aa 20‑201, 208‑393 and 400‑591), plus 15 intrachain disulfide bonds. Rat AFP contains an alternate start site at Met287 that may generate an intracellular 37 kDa form. Mouse AFP also shows an analogous site at Met281 that may produce an equivalent isoform. Over aa 19‑605, mouse AFP shares 83% and 66% aa identity with rat and human AFP, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.