alpha-Fetoprotein/AFP Antibody (AFP-11) - BSA Free

Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (AFP-11) [NB500-524] - Staining of hepatocellular carcinoma (fetal liver; paraffin-embedded sections)

Product Discontinued

alpha-Fetoprotein/AFP Antibody (AFP-11) - BSA Free Summary

• Immunogen: purified alpha-Fetoprotein
• Localization: Secreted
• Specificity: This antibody (clone AFP-11) reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AFP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Radioimmunoassay 1:100-1:2000
  • Western Blot 2 ug/ml
• Application Notes: ELISA: This antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody NB500-643. Immunohistochemistry (paraffin sections): Recommended dilution: 10 ug/ml; positive tissue: hepatocellular carcinoma; heat mediated antigen retrieval (sodium citrate buffer); mAb incubation 1 hour / RT, detection DAB. Western blotting: Recommended dilution: 2 ug/ml, non-reducing conditions.
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  alpha-Fetoprotein/AFP Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for alpha-Fetoprotein/AFP Antibody (AFP-11) - BSA Free

• AFP
• Alpha-1-fetoprotein
• alpha-fetoglobulin
• alpha-Fetoprotein
• DSCAM2
• FETA
• HP
• HPAFP

Background

Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AFP
• Entrez:
  • 174(Human)