alpha-Fetoprotein/AFP Antibody (870826) [Unconjugated]

Recombinant Mouse alpha ‑Fetoprotein/AFP protein was serially diluted 2-fold and captured by Rat Anti-Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB5369) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse alpha ‑Fetoprotein/AFP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5369) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Mu
• Applications: ELISA
• Clone: 870826
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

alpha-Fetoprotein/AFP Antibody (870826) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse alpha-Fetoprotein/AFP
  Lys19-Val605
  Accession # P02772
• Specificity: Detects mouse alpha-Fetoprotein in direct ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Fetoprotein/AFP Antibody (870826) [Unconjugated]

• AFP
• Alpha-1-fetoprotein
• alpha-fetoglobulin
• alpha-Fetoprotein
• DSCAM2
• FETA
• HP
• HPAFP

Background

AFP ( alpha -Fetoprotein) is a 69‑73 kDa member of the ALB/AFP/VDB family of proteins. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature mouse AFP is 587 amino acids (aa) in length. It contains three albumin domains (aa 20‑201, 208‑393 and 400‑591), plus 15 intrachain disulfide bonds. Rat AFP contains an alternate start site at Met287 that may generate an intracellular 37 kDa form. Mouse AFP also shows an analogous site at Met281 that may produce an equivalent isoform. Over aa 19‑605, mouse AFP shares 83% and 66% aa identity with rat and human AFP, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P02772()