alpha-Fetoprotein/AFP Antibody (10B8) - BSA Free

Product Discontinued

alpha-Fetoprotein/AFP Antibody (10B8) - BSA Free Summary

• Immunogen: Human alpha-fetoprotein isolated from amniotic fluid adsorbed onto aluminum hydroxide gel
• Epitope: Epitope belongs to group II a different group from Clone 06, -08 and -09.
• Specificity: Clone 10B8 is specific for human AFP. Cross-reactivity to other species or similar molecules have not been determined.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AFP
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
• Application Notes: Ka=190, determined as concentration of antibody giving half-maximal binding to hepatoma-AFP bound to solid phase (unit: 108 L/mol). Clone 10B8 reacts strongly with human AFP. A strong reaction is seen when tested in sandwich ELISA in combination with a polyclonal antibody against human AFP .

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for alpha-Fetoprotein/AFP Antibody (10B8) - BSA Free

• AFP
• Alpha-1-fetoprotein
• alpha-fetoglobulin
• alpha-Fetoprotein
• DSCAM2
• FETA
• HP
• HPAFP

Background

alpha-fetoprotein (AFP) is a serum protein produced by hepatocytes. AFP levels are used as a tumor marker for hepatomas and germ cell tumors, and the serum concentration has prognostic significance in other diseases. Levels of AFP is 0.6-9.3 kIU/L in blood from normal individuals under the age of 40 . Concentrations raise with age and during pregnancy.AFP consists of one single polypeptide chain of 590 aminoacids with MW 65 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AFP