alpha Adaptin Antibody (AP6) - BSA Free

Immunocytochemistry/Immunofluorescence: alpha Adaptin Antibody (AP6) [NB600-1545] - alpha Adaptin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/Immunofluorescence: alpha Adaptin Antibody (AP6) [NB600-1545] - alpha Adaptin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/Immunofluorescence: alpha Adaptin Antibody (AP6) [NB600-1545] - alpha Adaptin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

• Reactivity: Hu, Mu, Rt, Bv, Ha, Pm
• Applications: WB, Flow, ICC/IF, IHC, IP, B/N
• Clone: AP6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

alpha Adaptin Antibody (AP6) - BSA Free Summary

• Immunogen: Purified adaptors.
• Localization: Cytoplasmic
• Specificity: Detects assembly polypeptide 2 (AP2) It recognizes the products of both alpha-adaptin genes, alpha A and alpha C as well as an alternatively spliced isoform of alpha A found in neurons.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AP2A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry 1 ug / 10^6 cells
  • Immunocytochemistry/Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:200
• Application Notes: WB: Detects an approx. 100 kDa doublet representing the two isoforms of alpha-adaptin in rat brain, though alpha C is the dominant form seen. Microinjection of cells with this antibody results in variable blockade of endocytosis.

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 15923653). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for alpha Adaptin Antibody (AP6) - BSA Free

• 100 kDa coated vesicle protein A
• Adapter-related protein complex 2 alpha-1 subunit
• Adaptor protein complex AP-2 subunit alpha-1
• adaptor-related protein complex 2, alpha 1 subunit
• ADTAAAP2-ALPHA
• alpha1-adaptin
• Alpha-adaptin A
• AP-2 complex subunit alpha-1
• CLAPA1adaptin, alpha A
• Clathrin assembly protein complex 2 alpha-A large chain
• clathrin-associated/assembly/adaptor protein, large, alpha 1
• Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit

Background

AP2A1, also known as AP-2 complex subunit alpha-1, contains a 208 kDa and a 105 kDa isoform, and is involved in protein transport, vesicle formation, and clathrin-dependent endocytosis. Current research is being conducted on the relationship between AP2A1 and a variety of diseases and disorders, including seminoma, neuronitis, Huntington's disease, malaria, myeloid leukemia, carcinoma, gout, immunodeficiency, pancreatic cancer, prostatitis, prostate carcinoma, cholesterol, breast cancer, leukemia, and pancreatitis. AP2A1 is associated with Clathrin-dependent protein traffic, CTLA4 Signaling, p38 Signaling, the EGFR1 Signaling Pathway, the Synaptic Vesicle Pathway, the Arf1 pathway, and L1CAM interactions. The protein interacts with HIP1, TOE1, SMAD9, NUMB, and GRB2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AP2A1
• Entrez:
  • 160(Human)
  • 11771(Mouse)
  • 308578(Rat)