| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-721-100ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1545-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21875-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92798-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-721 | |||
| BSA Free | NB300-721-100ul | |||
![Immunocytochemistry/Immunofluorescence: alpha Adaptin Antibody (AC1-M11) [NB300-721] - Shows immunolocalization of alpha-adaptin in NRK cells.](http://images.novusbio.com/fullsize/alpha-Adaptin-Antibody-AC1-M11-Immunocytochemistry-Immunofluorescence-NB300-721-img0006.jpg "Immunocytochemistry/Immunofluorescence: alpha Adaptin Antibody (AC1-M11) [NB300-721] - Shows immunolocalization of alpha-adaptin in NRK cells.")
![Immunohistochemistry-Paraffin: alpha Adaptin Antibody (AC1-M11) [NB300-721] - Immunohistochemistry on deparaffinized human heart tissue](http://images.novusbio.com/fullsize/alpha-Adaptin-Antibody-AC1-M11-Immunohistochemistry-Paraffin-NB300-721-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Adaptin Antibody (AP6) [NB600-1545] - alpha Adaptin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/alpha-Adaptin-Antibody-AP6-Immunocytochemistry-Immunofluorescence-NB600-1545-img0003.jpg "Immunocytochemistry/Immunofluorescence: alpha Adaptin Antibody (AP6) [NB600-1545] - alpha Adaptin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunocytochemistry/Immunofluorescence: alpha Adaptin Antibody (AP6) [NB600-1545] - alpha Adaptin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/alpha-Adaptin-Antibody-AP6-Immunocytochemistry-Immunofluorescence-NB600-1545-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Adaptin Antibody (AP6) [NB600-1545] - alpha Adaptin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/alpha-Adaptin-Antibody-AP6-Immunocytochemistry-Immunofluorescence-NB600-1545-img0002.jpg)
![Western Blot: alpha Adaptin Antibody (8R8B6) [NBP3-16402] - Western blot analysis of extracts of various cell lines, using alpha Adaptin Rabbit mAb (NBP3-16402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/alpha-Adaptin-Antibody-8R8B6-Western-Blot-NBP3-16402-img0003.jpg "Western Blot: alpha Adaptin Antibody (8R8B6) [NBP3-16402] - Western blot analysis of extracts of various cell lines, using alpha Adaptin Rabbit mAb (NBP3-16402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: alpha Adaptin Antibody (8R8B6) [NBP3-16402] - Immunofluorescence analysis of C6 cells using alpha Adaptin Rabbit mAb (NBP3-16402) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/alpha-Adaptin-Antibody-8R8B6-Immunocytochemistry-Immunofluorescence-NBP3-16402-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Adaptin Antibody (8R8B6) [NBP3-16402] - Confocal imaging of NIH/3T3 cells using alpha Adaptin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16402_rabbit-alpha-adaptin-mab-8r8b6-5220251242522.jpg)
![Western Blot: alpha Adaptin Antibody (SR1044) [NBP3-21875] - Western blot analysis using NBP3-21875 in A431 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21875_rabbit-alpha-adaptin-mab-sr1044-572023162479.jpg "Western Blot: alpha Adaptin Antibody (SR1044) [NBP3-21875] - Western blot analysis using NBP3-21875 in A431 cell lysate.")
![Western Blot: alpha Adaptin Antibody (JE40-38) [NBP3-32040] - Western blot analysis of alpha Adaptin on different lysates with Rabbit anti-alpha Adaptin antibody (NBP3-32040) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 108 kDa Observed band size: 108 kDa Exposure time: 10 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32040) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32040_rabbit-alpha-adaptin-mab-je40-38-27820241631663.jpg "Western Blot: alpha Adaptin Antibody (JE40-38) [NBP3-32040] - Western blot analysis of alpha Adaptin on different lysates with Rabbit anti-alpha Adaptin antibody (NBP3-32040) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 108 kDa Observed band size: 108 kDa Exposure time: 10 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32040) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Adaptin Antibody (JE40-38) [NBP3-32040] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-alpha Adaptin antibody (NBP3-32040) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32040) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32040_rabbit-alpha-adaptin-mab-je40-38-298202413585544.jpg)
![Western Blot: alpha Adaptin Antibody [NBP2-92798] - Analysis of extracts of various cell lines, using alpha Adaptin at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/alpha-Adaptin-Antibody-Western-Blot-NBP2-92798-img0003.jpg "Western Blot: alpha Adaptin Antibody [NBP2-92798] - Analysis of extracts of various cell lines, using alpha Adaptin at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![SDS-Page: Recombinant Human alpha Adaptin Protein [H00000160-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Adaptin-Protein-SDS-Page-H00000160-Q01-img0001.jpg "SDS-Page: Recombinant Human alpha Adaptin Protein [H00000160-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunoprecipitation:alpha Adaptin AntibodyNBP3-29676] -Detection of human Alpha-adaptin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Alpha-adaptin antibody NBP3-29676 (lot NBP3-29676-1) used for IP at 6 µg per reaction. Alpha-adaptin was also immunoprecipitated by rabbit anti-Alpha-adaptin antibody for blotting immunoprecipitated Alpha-adaptin, NBP3-29676 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29676_rabbit-alpha-adaptin-pab-301220241556513.jpg "Immunoprecipitation:alpha Adaptin AntibodyNBP3-29676] -Detection of human Alpha-adaptin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Alpha-adaptin antibody NBP3-29676 (lot NBP3-29676-1) used for IP at 6 µg per reaction. Alpha-adaptin was also immunoprecipitated by rabbit anti-Alpha-adaptin antibody for blotting immunoprecipitated Alpha-adaptin, NBP3-29676 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: alpha Adaptin Antibody [NBP3-13154] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with alpha Adaptin antibody (NBP3-13154) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Adaptin-Antibody-Western-Blot-NBP3-13154-img0001.jpg "Western Blot: alpha Adaptin Antibody [NBP3-13154] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with alpha Adaptin antibody (NBP3-13154) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: alpha Adaptin Antibody [NBP1-68949] - Human Brain lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/alpha-Adaptin-Antibody-Western-Blot-NBP1-68949-img0002.jpg "Western Blot: alpha Adaptin Antibody [NBP1-68949] - Human Brain lysate, concentration 0.2-1 ug/ml.")