alpha Adaptin Antibody (AC1-M11)

Images

 
Immunocytochemistry/ Immunofluorescence: alpha Adaptin Antibody (AC1-M11) [NB300-721] - Shows immunolocalization of alpha-adaptin in NRK cells.
Immunohistochemistry-Paraffin: alpha Adaptin Antibody (AC1-M11) [NB300-721] - Immunohistochemistry on deparaffinized human heart tissue

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Am, Bv, Ch, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
AC1-M11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2 mg/ml

Order Details

alpha Adaptin Antibody (AC1-M11) Summary

Immunogen
Purified bovine brain adaptor complexes.
Localization
Cytoplasmic
Specificity
Detects Alpha 1 Fetoprotein. This detects the products of both alpha-adaptin genes, alpha A and alpha C, as well as an alternatively spliced isoform of alpha A found in neurons.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
AP2A1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Paraffin 1:20
  • Immunoprecipitation 1:10 - 1:500
Application Notes
WB: Detects an approx. 100 kDa doublet representing the two isoforms of Alpha 1 Fetoprotein from pig brain extract. IF: Staining of alpha-adaptin in transfected COS cells yields distinct punctate dots on the plasma membrane.
Reviewed Applications
Read 1 Review rated 4
using
NB300-721 in the following applications:

Publications
Read Publications using NB300-721.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
2 mg/ml
Purity
Protein A purified

Alternate Names for alpha Adaptin Antibody (AC1-M11)

  • 100 kDa coated vesicle protein A
  • Adapter-related protein complex 2 alpha-1 subunit
  • Adaptor protein complex AP-2 subunit alpha-1
  • adaptor-related protein complex 2, alpha 1 subunit
  • ADTAAAP2-ALPHA
  • alpha1-adaptin
  • Alpha-adaptin A
  • AP-2 complex subunit alpha-1
  • CLAPA1adaptin, alpha A
  • Clathrin assembly protein complex 2 alpha-A large chain
  • clathrin-associated/assembly/adaptor protein, large, alpha 1
  • Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Po, Am, Bv, Ch, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for alpha Adaptin Antibody (NB300-721)(2)

Review for alpha Adaptin Antibody (NB300-721) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB300-721:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot alpha Adaptin NB300-721
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reviewed by:
Anonymous
WB Human 01/18/2012
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Summary

ApplicationWestern Blot
Sample TestedA549 lung cancer cell line, Sample Amount: 10ug
SpeciesHuman
CommentsGood antibody

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 2 hours

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 2 hours

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse, Secondary Ab Dilution Ratio: 1:1000

Details

Detection NotesDetection Method: ECL blue film, Exposure Time: 20 minutes, Specific Bands: 95 kDa, Non-Specific Bands: ~ 100 kDa

Comments

CommentsGood antibody

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha Adaptin Antibody (NB300-721) (0)

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Secondary Antibodies

 

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Recent Reviews

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1
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Anonymous
01/18/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol AP2A1
Entrez
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