alpha Adaptin Antibody (AC1-M11) - BSA Free

Immunocytochemistry/Immunofluorescence: alpha Adaptin Antibody (AC1-M11) [NB300-721] - Shows immunolocalization of alpha-adaptin in NRK cells.

Immunohistochemistry-Paraffin: alpha Adaptin Antibody (AC1-M11) [NB300-721] - Immunohistochemistry on deparaffinized human heart tissue

• Reactivity: Hu, Mu, Rt, Po, Am, Bv, Ch, Pm
• Applications: WB, ICC/IF, IHC, IP
• Clone: AC1-M11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 2 mg/ml

alpha Adaptin Antibody (AC1-M11) - BSA Free Summary

• Description: Novus Biologicals Mouse alpha Adaptin Antibody (AC1-M11) - BSA Free (NB300-721) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-alpha Adaptin Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified bovine brain adaptor complexes.
• Localization: Cytoplasmic
• Specificity: Detects Alpha 1 Fetoprotein. This detects the products of both alpha-adaptin genes, alpha A and alpha C, as well as an alternatively spliced isoform of alpha A found in neurons.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: AP2A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Paraffin 1:20
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100
• Application Notes: WB: Detects an approx. 100 kDa doublet representing the two isoforms of Alpha 1 Fetoprotein from pig brain extract. IF: Staining of alpha-adaptin in transfected COS cells yields distinct punctate dots on the plasma membrane.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for alpha Adaptin Antibody (AC1-M11) - BSA Free

• 100 kDa coated vesicle protein A
• Adapter-related protein complex 2 alpha-1 subunit
• Adaptor protein complex AP-2 subunit alpha-1
• adaptor-related protein complex 2, alpha 1 subunit
• ADTAAAP2-ALPHA
• alpha1-adaptin
• Alpha-adaptin A
• AP-2 complex subunit alpha-1
• CLAPA1adaptin, alpha A
• Clathrin assembly protein complex 2 alpha-A large chain
• clathrin-associated/assembly/adaptor protein, large, alpha 1
• Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit

Background

AP2A1, also known as AP-2 complex subunit alpha-1, contains a 208 kDa and a 105 kDa isoform, and is involved in protein transport, vesicle formation, and clathrin-dependent endocytosis. Current research is being conducted on the relationship between AP2A1 and a variety of diseases and disorders, including seminoma, neuronitis, Huntington's disease, malaria, myeloid leukemia, carcinoma, gout, immunodeficiency, pancreatic cancer, prostatitis, prostate carcinoma, cholesterol, breast cancer, leukemia, and pancreatitis. AP2A1 is associated with Clathrin-dependent protein traffic, CTLA4 Signaling, p38 Signaling, the EGFR1 Signaling Pathway, the Synaptic Vesicle Pathway, the Arf1 pathway, and L1CAM interactions. The protein interacts with HIP1, TOE1, SMAD9, NUMB, and GRB2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha Adaptin Antibody (NB300-721)(2)

Publications using NB300-721

• Rudinskiy N, Grishchuk Y, Vaslin A et al. Calpain hydrolysis of alpha- beta2-adaptins decreases clathrin-dependent endocytosis may promote neurodegeneration. J Biol Chem;284(18):12447-58. 2009-05-01 [PMID: 19240038]
• Robinson MS 100-kD coated vesicle proteins molecular heterogeneity intracellular distribution studied with monoclonal antibodies. J Cell Biol 104:887-95. 1987-01-01 [PMID: 2881934]

Review for alpha Adaptin Antibody (NB300-721) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/18/2012

Summary

• Application: Western Blot
• Sample Tested: A549 lung cancer cell line, Sample Amount: 10ug
• Species: Human

Bioinformatics

• Gene Symbol: AP2A1
• Entrez:
  • 160(Human)
  • 11771(Mouse)
  • 308578(Rat)