alpha Adaptin Antibody (AC1-M11) [Alexa Fluor® 350]

• Reactivity: Hu, Mu, Rt, Po, Am, Bv, Ch, Pm
• Applications: WB, ICC/IF, IHC, IP
• Clone: AC1-M11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

alpha Adaptin Antibody (AC1-M11) [Alexa Fluor® 350] Summary

• Immunogen: Purified bovine brain adaptor complexes.
• Localization: Cytoplasmic
• Specificity: Detects Alpha 1 Fetoprotein. This detects the products of both alpha-adaptin genes, alpha A and alpha C, as well as an alternatively spliced isoform of alpha A found in neurons.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: AP2A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for alpha Adaptin Antibody (AC1-M11) [Alexa Fluor® 350]

• 100 kDa coated vesicle protein A
• Adapter-related protein complex 2 alpha-1 subunit
• Adaptor protein complex AP-2 subunit alpha-1
• adaptor-related protein complex 2, alpha 1 subunit
• ADTAAAP2-ALPHA
• alpha1-adaptin
• Alpha-adaptin A
• AP-2 complex subunit alpha-1
• CLAPA1adaptin, alpha A
• Clathrin assembly protein complex 2 alpha-A large chain
• clathrin-associated/assembly/adaptor protein, large, alpha 1
• Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit

Background

AP2A1, also known as AP-2 complex subunit alpha-1, contains a 208 kDa and a 105 kDa isoform, and is involved in protein transport, vesicle formation, and clathrin-dependent endocytosis. Current research is being conducted on the relationship between AP2A1 and a variety of diseases and disorders, including seminoma, neuronitis, Huntington's disease, malaria, myeloid leukemia, carcinoma, gout, immunodeficiency, pancreatic cancer, prostatitis, prostate carcinoma, cholesterol, breast cancer, leukemia, and pancreatitis. AP2A1 is associated with Clathrin-dependent protein traffic, CTLA4 Signaling, p38 Signaling, the EGFR1 Signaling Pathway, the Synaptic Vesicle Pathway, the Arf1 pathway, and L1CAM interactions. The protein interacts with HIP1, TOE1, SMAD9, NUMB, and GRB2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AP2A1