Alpha Actinin 2 Antibody - BSA Free

Western Blot: Alpha Actinin 2 Antibody [NBP2-15355] - Alpha Actinin 2 antibody detects Alpha Actinin 2 protein by western blot analysis. Rat tissue extracts (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody diluted at 1:5000.

Immunocytochemistry/Immunofluorescence: Alpha Actinin 2 Antibody [NBP2-15355] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP2-15355] - Alpha Actinin 2 antibody detects Alpha Actinin 2 protein at cytoplasm and membrane on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Alpha Actinin 2 antibody diluted at 1:250.

Western Blot: Alpha Actinin 2 Antibody [NBP2-15355] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7. 5% SDS PAGE, antibody diluted at 1:3000.

Western Blot: Alpha Actinin 2 Antibody [NBP2-15355] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.

Western Blot: Alpha Actinin 2 Antibody [NBP2-15355] -Mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP2-15355] -Analysis of paraffin-embedded zebrafish tissue, using alpha Actinin 2 antibody [N2C1], Internal at 1:300 dilution.

Western Blot: Alpha Actinin 2 Antibody [NBP2-15355] - Sample (10 ug of whole cell lysate) A: adult zebrafish 7.5% SDS PAGE NBP2-15355 diluted at 1:3000

• Reactivity: Hu, Mu, Rt, Ze
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Alpha Actinin 2 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Alpha Actinin 2. The exact sequence is proprietary.
• Localization: Cytoplasm; myofibril; sarcomere; Z-disk
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACTN2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 104 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Alpha Actinin 2 Antibody - BSA Free

• Actinin alpha 2
• actinin, alpha 2
• Actinin-a2
• ACTN2
• alpha Actinin 2
• alpha-Actinin 2
• Alpha-actinin skeletal muscle isoform 2
• Alpha-Actinin Skeletal Muscle
• Alpha-Actinin-2
• CMD1AA
• F-actin cross-linking protein

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ACTN2
• Uniprot:
  • P35609()