alpha 1-Microglobulin Antibody

Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human a1-Microglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7724) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a1-Microglobulin at approximately 30-32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

alpha 1-Microglobulin Antibody Summary

• Immunogen: E. coli-derived recombinant human alpha 1-Microglobulin
  Gly20-Val203, predicted
  Accession # P02760
• Specificity: Detects human alpha 1-Microglobulin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant rat alpha 1-Microglobulin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: AMBP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha 1-Microglobulin Antibody

• A1M
• alpha 1Microglobulin
• alpha 1-Microglobulin
• alpha-1-microglobulin/bikunin precursor
• AMBP
• bikunin
• EDC1
• HCP
• HCPcomplex-forming glycoprotein heterogeneous in charge
• HI30
• IATIL
• inter-alpha-trypsin inhibitor light chain
• ITI
• ITIL
• ITILC
• ITILgrowth-inhibiting protein 19
• protein AMBP
• protein HC
• trypstatin
• uristatin
• uronic-acid-rich protein
• UTI

Background

Human alpha 1-Microglobulin (alpha1-m/A1M; also protein HC) is a secreted, 31-32 kDa glycoprotein member of the lipocalin family, calycin superfamily of molecules. It is expressed by hepatocytes, keratinocytes, and endodermal derivatives in the embryo. A1M appears to act as a heme scavenger, protecting cells and collagen against oxidative damage. It also acts as an immunosuppressant, inhibiting polyclonal lymphocyte activation and dampening granulocyte migration in response to chemokines. A1M circulates either as a monomer, or bound to IgA, albumin or prothrombin. Human A1M is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP-1, a 120-140 kDa adrenomedullin-binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an N-terminal 183 aa A1M protein (aa 20-203), and a C-terminal serine protease inhibitor termed bikunin (aa 206-352). A1M possesses one lipocalin domain (aa 42-186). Although cleavage of AMBP in the Golgi apparatus typically generates a 31 kDa A1M and 28 kDa bikunin molecule, the 60-65 kDa AMBP precursor can also be released intact. A1M will undergo extracellular processing, generating a 30 kDa isoform that is missing aa 199-203. There is one splice variant that shows a deletion of aa 48-57. Over aa 20-203, human A1M shares 76% aa sequence identity with both mouse and rat A1M.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AMBP
• Uniprot:
  • P02760(Human)