ALOX12B Antibody - Azide and BSA Free

Western Blot: ALOX12B Antibody [NBP3-04581] - Western blot analysis of extracts of Rat testis, using ALOX12B antibody (NBP3-04581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Western Blot: ALOX12B Antibody [NBP3-04581] - Western blot analysis of extracts of various cell lines, using ALOX12B antibody (NBP3-04581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ALOX12B Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit ALOX12B Antibody - Azide and BSA Free (NBP3-04581) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ALOX12B (NP_001130.1). MATYKVRVATGTDLLSGTRDSISLTIVGTQGESHKQLLNHFGRDFATGAVGQYTVQCPQDLGELIIIRLHKERYAFFPKDPWYCNYVQICAPNGRIYHFPAYQWMDGYETLALREATGKTTADDSLPVLLEHRKEEIRAKQDFYHWRVFLPGLPSYVHIPSYRPPVRRHRNPNRPEWNGYIPGFPILINFKATKFLNLNLRYSFLKTASFFVRLGPMALAFKVRGLLDCKHSWKRLKDIRKIFPGKKSVV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALOX12B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for ALOX12B Antibody - Azide and BSA Free

• 12R-LOXEC 1.13.11.-
• arachidonate 12-lipoxygenase, 12R type
• arachidonate 12-lipoxygenase, 12R-type
• EC 1.13.11.33,12R-lipoxygenase
• Epidermis-type lipoxygenase 12

Background

ALOX12B, or Arachidonate 12-lipoxygenase, 12R-type has a 701 amino acid isoform that is 80 kDa, and is involved in the transformation of arachidonic acid into 12R-hydroxyeicosatetraenoic acid. Current research is being studied on the relation between ALOX12B and a variety of diseases and disorders including epithelial ovarian cancer, meningioma, breast cancer, leukemia, tonsillitis, ichthyosis, anhidrosis, autosomal recessive congenital ichthyosis, and dermatitis. This protein interacts with LMNA, KRT5, ALOX12, ALOX15, and ALOX15B in arachidonic acid metabolism, 112-eicosatetraenoic acid derivative synthesis, and lipid and lipoprotein metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: ALOX12B