ALMS1 Antibody

Western Blot: ALMS1 Antibody [NB100-97823] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-97823 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. ALMS1 was also immunoprecipitated by NB100-97824, which recognizes a downstream epitope.

Immunocytochemistry/Immunofluorescence: ALMS1 Antibody [NB100-97823] - NBF-fixed HeLa cell. Antibody used at a dilution of 1:1,000 (0.2ug/ml).

Immunocytochemistry/Immunofluorescence: ALMS1 Antibody [NB100-97823] - NBF-fixed asynchronous HeLa cells. At a dilution of 1:250.

Immunocytochemistry/Immunofluorescence: ALMS1 Antibody [NB100-97823] - NBF-fixed HeLa cell. Antibody used at a dilution of 1:1,000 (0.2ug/ml).

Product Discontinued

ALMS1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1200 and 1250 of human Alstrom syndrome 1 using the numbering given in entry EAW99731.1 (GeneID 7840).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALMS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: ICC-IF- Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ALMS1 Antibody

• Alstrom syndrome 1
• Alstrom syndrome protein 1
• DKFZp686A118
• DKFZp686D1828
• KIAA0328ALSS

Background

Mutations in the ALMS1 gene are the cause of Alstrom syndrome, a rare autosomal recessive disease characterized by type 2 diabetes mellitus, obesity, cardiomyopathy, and neurosensory defects such as cone-rod retinal dystrophy, and hearing loss. In the cell, ALMS1 localizes to basal bodies of ciliated cells and centrosomes and is thus a member of proteins associated with genetic ciliopathies. ALMS1 is also known as KIAA0328 and ALSS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALMS1 Antibody (NB100-97823)(1)

Publication using NB100-97823

• Schreyer E, Obringer C, Messaddeq N et al. PATAS, a First-in-Class Therapeutic Peptide Biologic, Improves Whole-Body Insulin Resistance and Associated Comorbidities In Vivo Diabetes 2022-07-13 [PMID: 35822820]

Bioinformatics

• Gene Symbol: ALMS1
• Entrez:
  • 7840(Human)
• Uniprot:
  • D6W5H5(Human)