Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) - BSA Free

Flow Cytometry: Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) [NBP3-09106] - Flow cytometry surface staining pattern of human peripheral whole blood spiked with HeLa cells stained using anti-TNAP (W8B2B10) purified antibody (concentration in sample 0.3 ug/ml).

Flow Cytometry: Mouse Monoclonal Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) [NBP3-09106] - Anti-Hu TNAP purified antibody (concentration in sample 0.3 ug/ml, GAM APC, red-filled histogram) binds specifically to TNAP on subpopulation of HeLa cells (upper panel), but not to surface of K562 cells (lower panel). Level of non-specific binding was assessed using cells unstained by primary antibody (GAM APC, black-dashed histogram).

• Reactivity: Hu, Po, Ca, Gt, Pm, Sh
• Applications: Flow, ICC/IF
• Clone: W8B2B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) - BSA Free Summary

• Immunogen: WERI-RB-1 retinoblastoma cell line
• Specificity: The mouse monoclonal antibody W8B2B10 recognizes TNAP (tissue non-specific alkaline phosphatase), an ectoenzyme expressed mainly on embryonic stem cells, liver, bone, and kidney cells. This antibody is suitable for characterization of bone marrow-derived MSCs, iPSCs, and ESCs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALPL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-4 ug/ml
  • Immunocytochemistry/ Immunofluorescence

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Alkaline Phosphatase, Tissue Non-Specific Antibody (W8B2B10) - BSA Free

• Alkaline phosphatase liver/bone/kidney isozyme
• alkaline phosphatase, liver/bone/kidney
• alkaline phosphatase, tissue-nonspecific isozyme
• alkaline phosphomonoesterase
• APTNAP
• AP-TNAP
• EC 3.1.3.1
• FLJ40094
• FLJ93059
• glycerophosphatase
• HOPS
• liver/bone/kidney-type alkaline phosphatase
• MGC161443
• tissue-nonspecific ALP
• TNAP
• TNSALPMGC167935

Background

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALPL