Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1)

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1) [NBP2-44962] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1).

• Reactivity: Hu, Bv
• Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, ICC/IF
• Clone: V17.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1) Summary

• Immunogen: Bovine alkaline phosphatase (Uniprot: P05186)
• Localization: Cell Surface
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALPL
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1-2 ug/ml
  • Immunohistochemistry-Paraffin
• Application Notes: Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47996)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1)

• Alkaline phosphatase liver/bone/kidney isozyme
• alkaline phosphatase, liver/bone/kidney
• alkaline phosphatase, tissue-nonspecific isozyme
• alkaline phosphomonoesterase
• APTNAP
• AP-TNAP
• EC 3.1.3.1
• FLJ40094
• FLJ93059
• glycerophosphatase
• HOPS
• liver/bone/kidney-type alkaline phosphatase
• MGC161443
• tissue-nonspecific ALP
• TNAP
• TNSALPMGC167935

Background

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALPL
• Uniprot:
  • P05186()
  • P05186()
  • P05187()