Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49)

Images

 
Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) [NB600-540] - Confocal fluorescent analysis of A431 using anti-TRA-2-49 Mouse monoclonal antibody (Red). Actin ...read more
Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) [NB600-540] - Analysis using anti-TRA-2-49. 2102Ep cells were stained with anti-TRA-2-49 antibody (Green) or isotype control antibody (grey).

Product Details

Summary
Reactivity Hu, Po, Fe, RbSpecies Glossary
Applications Flow, ICC/IF, IP
Clone
TRA-2-49
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) Summary

Immunogen
2102Ep Human Embryonal Carcinoma Cells
Epitope
Liver/Bone/Kidney Alkaline Phosphatase
Localization
Cell Membrane
Specificity
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used in flow cytometry to determine the expression of the Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence monitor the differentiation status of EC and ES cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ALPL
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 5-20 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
Does not work on frozen sections.
Positive Control
Alkaline Phosphatase, Tissue Non-Specific Lysate (NBL1-07487)
Publications
Read Publication using
NB600-540 in the following applications:

Reactivity Notes

Does not react with Guinea Pig, Rat, Mouse, Monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM PBS (pH 7.6) and 0.25M NaCl
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49)

  • Alkaline phosphatase liver/bone/kidney isozyme
  • alkaline phosphatase, liver/bone/kidney
  • alkaline phosphatase, tissue-nonspecific isozyme
  • alkaline phosphomonoesterase
  • APTNAP
  • AP-TNAP
  • EC 3.1.3.1
  • FLJ40094
  • FLJ93059
  • glycerophosphatase
  • HOPS
  • liver/bone/kidney-type alkaline phosphatase
  • MGC161443
  • tissue-nonspecific ALP
  • TNAP
  • TNSALPMGC167935

Background

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Alkaline Phosphatase, Tissue Non-Specific Antibody (NB600-540)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: FLOW.


Filter By Application
FLOW
(1)
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Filter By Species
Human
(1)
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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Alkaline Phosphatase, Tissue Non-Specific Antibody (NB600-540). (Showing 1 - 1 of 1 FAQ).

  1. Please advice if NB600-540 Alkaline Phosphatase Antibody can be used for IHC-P ?
    • This antibody has not yet been tested in IHC on paraffin embedded tissues. This antibody has only been validated in Flow, ICC, and IP. If you would like to try this antibody in IHC, we can offer our Innovator's Reward Program. Otherwise, we have the following products available to this target that are validated in IHC with paraffin-embedded tissues: View roducts.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ALPL
Entrez