Alkaline Phosphatase, Intestinal Native Protein

• Reactivity: Bv
• Applications: ELISA
• Concentration: 15 mg/ml

Alkaline Phosphatase, Intestinal Native Protein Summary

• Description: Native calf alkaline phosphatase.
  

  Source: Calf intestine

  Amino Acid Sequence: UniProt P19111
  4000 U/mg protein. 1 unit liberates 1 umole of p-nitrophenol per minute at 37C and pH 9.8 in DEA buffer. Activity is determined by measuring the increase in the optical density at 405 nm resulting from the hydrolysis of p-nitrophenyl phosphate.

• Preparation Method: Purified AP from calf intestine - liquid. Crystalline suspension. Free of endonuclease, exonuclease and RNase activities.
• Protein/Peptide Type: Native Protein
• Gene: ALPI

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Activity: 4000 U/mg protein.1 unit liberates 1 umole of p-nitrophenol per minute at 37C and pH 9.8 in DEA buffer. Activity is determined by measuring the increase in the optical density at 405nm resulting from the hydrolysis of p-nitrophenyl phosphate.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 70% Ammonium sulphate
• Preservative: No Preservative
• Concentration: 15 mg/ml

Alternate Names for Alkaline Phosphatase, Intestinal Native Protein

• alkaline phosphatase, intestinal
• Alkaline phosphomonoesterase
• ALPI
• EC 3.1.3.1
• Glycerophosphatase
• IAP
• Intestinal alkaline phosphatase
• intestinal-type alkaline phosphatase
• Kasahara isozyme

Background

Alkaline phosphatase removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes - placental, placental-like, intestinal and non-tissue specific (found in liver, kidney and bone).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ALPI
• Uniprot:
  • P19111(Bovine)