Rat Alkaline Phosphatase, Intestinal ELISA Kit (Colorimetric)

Rat Alkaline Phosphatase, Intestinal ELISA Kit (Colorimetric) [NBP2-68196] - Standard Curve Reference

ELISA: Rat Alkaline Phosphatase, Intestinal ELISA Kit (Colorimetric) [NBP2-68196] - Samples were spiked with high concentrations of Rat Alkaline Phosphatase, Intestinal and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Alkaline Phosphatase, Intestinal ELISA Kit (Colorimetric) [NBP2-68196] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL

Rat Alkaline Phosphatase, Intestinal ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat ALPI in samples. No significant cross-reactivity or interference between Rat ALPI and analogues was observed.
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.8%
• Intra-Assay: CV% < 4.7%
• Spike Recovery: 88-106%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ALPI

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Alkaline Phosphatase, Intestinal ELISA Kit (Colorimetric)

• alkaline phosphatase, intestinal
• Alkaline phosphomonoesterase
• ALPI
• EC 3.1.3.1
• Glycerophosphatase
• IAP
• Intestinal alkaline phosphatase
• intestinal-type alkaline phosphatase
• Kasahara isozyme

Background

Alkaline phosphatase removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes - placental, placental-like, intestinal and non-tissue specific (found in liver, kidney and bone).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Alkaline Phosphatase, Intestinal ELISA Kit (NBP2-68196)(2)

Publications using NBP2-68196

• Wang Z, Jiang X, Zhang L, Chen H Protective effects of Althaea officinalis L. extract against N ?diethylnitrosamine?induced hepatocellular carcinoma in male Wistar rats through antioxidative, anti?inflammatory, mitochondrial apoptosis and PI3K /Akt/ mTOR signaling pathways Food Science & Nutrition 2023-05-26 (ELISA)
• Zhenqian Wang, Xiao Jiang, Long Zhang, Han Chen Protective effects of Althaea officinalis L. extract against N ‐diethylnitrosamine‐induced hepatocellular carcinoma in male Wistar rats through antioxidative, anti‐inflammatory, mitochondrial apoptosis and PI3K /Akt/ mTOR signaling pathways Food Science & Nutrition 2023-05-26 [PMID: 37576045]

FAQs for Alkaline Phosphatase, Intestinal ELISA Kit (NBP2-68196). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ALPI