Alkaline Phosphatase, Intestinal Antibody

Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB600-588] - Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops.

Product Discontinued

Alkaline Phosphatase, Intestinal Antibody Summary

• Description: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Alkaline Phosphatase [Human Intestine]
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Alkaline Phosphatase, Intestinal, Intestinal [Human Intestine] (Uniprot: P09923)
• Specificity: Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined.
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALPI
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:20000
  • Western Blot 1:500-1:2000
• Application Notes: This product has been tested by western blot and is suitable to be assayed against 1.0 ug of Alkaline Phosphatase [Human Intestine] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
• Control:

  Alkaline Phosphatase, Tissue Non-Specific Overexpression Lysate

Reactivity Notes

Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Delipidation and Defibrination

Alternate Names for Alkaline Phosphatase, Intestinal Antibody

• alkaline phosphatase, intestinal
• Alkaline phosphomonoesterase
• ALPI
• EC 3.1.3.1
• Glycerophosphatase
• IAP
• Intestinal alkaline phosphatase
• intestinal-type alkaline phosphatase
• Kasahara isozyme

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Alkaline Phosphatase, Intestinal Antibody (NB600-588)(2)

Publications using NB600-588

• Papetti M, Augenlicht LH. MYBL2, downregulated during colon epithelial cell maturation, is suppressed by miR-365. Am J Physiol Gastrointest Liver Physiol. 2011-07-07 [PMID: 21737779]
• Wilson AJ, Byun DS, Popova N et al. Histone deacetylase 3 (HDAC3) and other class I HDACs regulate colon cell maturation and p21 expression and are deregulated in human colon cancer. J Biol Chem. 2006-05-12 [PMID: 16533812]

Bioinformatics

• Gene Symbol: ALPI
• Entrez:
  • 248(Human)
• Uniprot:
  • P09923(Human)