Alkaline Phosphatase, Intestinal Antibody

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Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB600-588] - Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Alkaline Phosphatase, Intestinal Antibody Summary

Description
This antibody was prepared from monospecific antiserum by a delipidation and defibrination.
Immunogen
Alkaline Phosphatase [human intestine]
Specificity
This polyclonal reacts with alkaline phosphatase from human intestine showing a 60 kD band against this enzyme under reducing conditions of SDS-PAGE. Elevated expression of this enzyme is associated with undifferentiated pluripoten stem cell (PSC).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALPI
Purity
Delipidation and Defibrination
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:5000-1:20000
Application Notes
This product has been assayed against 1.0 ug of Alkaline Phosphatase [Human Intestine] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:5,000 - 1:20,000 of the reconstitution concentration is suggested for this product.
Positive Control
Alkaline Phosphatase, Tissue Non-Specific Lysate (NBL1-07487)
Publications
Read Publications using NB600-588.

Reactivity Notes

Human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Delipidation and Defibrination

Alternate Names for Alkaline Phosphatase, Intestinal Antibody

  • alkaline phosphatase, intestinal
  • Alkaline phosphomonoesterase
  • ALPI
  • EC 3.1.3.1
  • Glycerophosphatase
  • IAP
  • Intestinal alkaline phosphatase
  • intestinal-type alkaline phosphatase
  • Kasahara isozyme

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Alkaline Phosphatase, Intestinal Antibody (NB600-588)(2)

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol ALPI
Entrez