Alkaline Phosphatase, Intestinal Antibody - Azide and BSA Free

• Reactivity: Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Alkaline Phosphatase, Intestinal Antibody - Azide and BSA Free Summary

• Description: This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine]
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Alkaline Phosphatase, Intestinal, Intestinal [Calf Intestine] (Uniprot: P19111)
• Localization: Attached to the membrane by a GPI-anchor.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ALPI
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:4000-1:20000
  • Western Blot 1:500-1:2000
• Application Notes: This product has been assayed against 1.0 ug of Alkaline Phosphatase [Calf intestine] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Cross reactivity against Alkaline Phosphatase, Intestinal from other tissues and species may occur but have not been specifically determined.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: No Preservative
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Alkaline Phosphatase, Intestinal Antibody - Azide and BSA Free

• alkaline phosphatase, intestinal
• Alkaline phosphomonoesterase
• ALPI
• EC 3.1.3.1
• Glycerophosphatase
• IAP
• Intestinal alkaline phosphatase
• intestinal-type alkaline phosphatase
• Kasahara isozyme

Background

Alkaline phosphatase removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes - placental, placental-like, intestinal and non-tissue specific (found in liver, kidney and bone).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Alkaline Phosphatase, Intestinal Antibody (NB120-7328). (Showing 1 - 1 of 1 FAQ).

1. I recently received the Alkaline Phosphatase (calf intestinal) antibody from Novus (Cat# NB120-7328, Lot# 170) and in initial experiments it works nicely. However, I would like to use it for quantitative experiments. Therefore, it would be very helpful if you could tell me the molar concentration of this antibody.
   • The concentration of Lot 170 is 1mg/ml.

Bioinformatics

• Gene Symbol: ALPI
• Entrez:
  • 248(Human)
• Uniprot:
  • P19111(Bovine)