Alkaline Phosphatase, Intestinal Antibody

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Product Details

Summary
Reactivity BvSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

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Catalog# & Conjugate Size Price

Alkaline Phosphatase, Intestinal Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Alkaline Phosphatase [Calf intestine]
Localization
Attached to the membrane by a GPI-anchor.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ALPI
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:4000-1:20000

Reactivity Notes

Cross-reacts with Cow. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
No Preservative
Purity
Ion exchange chromatography

Alternate Names for Alkaline Phosphatase, Intestinal Antibody

  • alkaline phosphatase, intestinal
  • Alkaline phosphomonoesterase
  • ALPI
  • EC 3.1.3.1
  • Glycerophosphatase
  • IAP
  • Intestinal alkaline phosphatase
  • intestinal-type alkaline phosphatase
  • Kasahara isozyme

Background

Alkaline phosphatase removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes - placental, placental-like, intestinal and non-tissue specific (found in liver, kidney and bone).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Alkaline Phosphatase, Intestinal Antibody (NB120-7328). (Showing 1 - 1 of 1 FAQ).

  1. I recently received the Alkaline Phosphatase (calf intestinal) antibody from Novus (Cat# NB120-7328, Lot# 170) and in initial experiments it works nicely. However, I would like to use it for quantitative experiments. Therefore, it would be very helpful if you could tell me the molar concentration of this antibody.
    • The concentration of Lot 170 is 1mg/ml.

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Bioinformatics

Gene Symbol ALPI
Entrez