Alkaline Phosphatase/ALPP Antibody (P16-D)

Images

 
Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (P16-D) [NBP1-30149] - Diffuse cytoplasmic PLAP positivity in the typical seminoma. Formalin fixed, paraffin embedded human tissue (4 um section) stained ...read more
Immunohistochemistry-Paraffin: Alkaline Phosphatase, Placental Antibody (P16-D) [NBP1-30149] - human seminoma stained with anti-Placental Alkaline Phosphatase antibody shows strong positive membrane immunostaining of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-Fr, IHC-P
Clone
P16-D
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Alkaline Phosphatase/ALPP Antibody (P16-D) Summary

Immunogen
Peptide derived from the middle portion of human PLAP sequence.
Specificity
This antibody is specific to an internal portion of human PLAP sequence.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ALPP
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100-200
Application Notes
For use in Immunohistochemistry frozen and paraffin embedded sections. For 0.1ml, 0.5ml, and 1.0ml sizes, use recommended dilutions. For 7.0ml and 15.0ml sizes, products should be used undiluted.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
20mM Tris/HCl (pH 8.0) and 20 mg/ml BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

The 7mL and 15mL sizes that this product is available in is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Alternate Names for Alkaline Phosphatase/ALPP Antibody (P16-D)

  • Alkaline phosphatase Regan isozyme
  • Alkaline Phosphatase
  • alkaline phosphatase, placental (Regan isozyme)
  • alkaline phosphatase, placental type
  • alkaline phosphatase, placental
  • alkaline phosphomonoesterase
  • ALP
  • ALPP
  • EC 3.1.3.1
  • FLJ61142
  • glycerophosphatase
  • PALP
  • Placental alkaline phosphatase 1
  • PLAP
  • PLAP-1
  • Regan isozyme
  • SEAP

Background

Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer. HD 3F6 has potential uses in the diagnosis of ovarian and testicular tumours. The antibody detects both Regan and Nagao isoenzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for Alkaline Phosphatase/ALPP Antibody (NBP1-30149). (Showing 1 - 1 of 1 FAQ).

  1. I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?
    • None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.

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Bioinformatics

Gene Symbol ALPP
Entrez
Uniprot