Alkaline Phosphatase/ALPP Antibody (P16-D)

Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (P16-D) [NBP1-30149] - Diffuse cytoplasmic PLAP positivity in the typical seminoma. Formalin fixed, paraffin embedded human tissue (4 um section) stained with anti-PLAP antibody.

Product Discontinued

Alkaline Phosphatase/ALPP Antibody (P16-D) Summary

• Description: This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Peptide derived from the middle portion of human Alkaline Phosphatase/ALPP sequence. Antibody recognizes the epitope between Glu238 - Leu250.
• Epitope: Glu238 - Leu250
• Specificity: This antibody is specific to an internal portion of human PLAP sequence.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ALPP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-200

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 20mM Tris-HCl (pH 8.0) and 20mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Alternate Names for Alkaline Phosphatase/ALPP Antibody (P16-D)

• Alkaline phosphatase Regan isozyme
• Alkaline Phosphatase
• alkaline phosphatase, placental (Regan isozyme)
• alkaline phosphatase, placental type
• alkaline phosphatase, placental
• alkaline phosphomonoesterase
• ALP
• ALPP
• EC 3.1.3.1
• FLJ61142
• glycerophosphatase
• PALP
• Placental alkaline phosphatase 1
• PLAP
• PLAP-1
• Regan isozyme
• SEAP

Background

Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer. HD 3F6 has potential uses in the diagnosis of ovarian and testicular tumours. The antibody detects both Regan and Nagao isoenzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Alkaline Phosphatase/ALPP Antibody (NBP1-30149). (Showing 1 - 1 of 1 FAQ).

1. I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?
   • None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.

Bioinformatics

• Gene Symbol: ALPP
• Entrez:
  • 250 (Human)
• Uniprot:
  • P05187()