Alkaline Phosphatase/ALPP Antibody (HD 11F7) [Alexa Fluor® 532]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IHC, IHC-Fr, IHC-P
Clone
HD 11F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 532

Alkaline Phosphatase/ALPP Antibody (HD 11F7) [Alexa Fluor® 532] Summary

Immunogen
Fresh Hep-2 cells followed by booster injections of fresh Hep-2 cells that had their surface expression of PLAP boosted in culture.
Specificity
This antibody recognizes both Regan and Nagao isoenzymes of placental alkaline phosphatase (PLAP).
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ALPP
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Alkaline Phosphatase/ALPP Antibody (HD 11F7) [Alexa Fluor® 532]

  • Alkaline phosphatase Regan isozyme
  • Alkaline Phosphatase
  • alkaline phosphatase, placental (Regan isozyme)
  • alkaline phosphatase, placental type
  • alkaline phosphatase, placental
  • alkaline phosphomonoesterase
  • ALP
  • ALPP
  • EC 3.1.3.1
  • FLJ61142
  • glycerophosphatase
  • PALP
  • Placental alkaline phosphatase 1
  • PLAP
  • PLAP-1
  • Regan isozyme
  • SEAP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for Alkaline Phosphatase/ALPP Antibody (NBP2-76654AF532). (Showing 1 - 1 of 1 FAQ).

  1. I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?
    • None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-76654AF350
Alexa Fluor 405 NBP2-76654AF405
Alexa Fluor 488 NBP2-76654AF488
Alexa Fluor 532 NBP2-76654AF532
Alexa Fluor 594 NBP2-76654AF594
Alexa Fluor 647 NBP2-76654AF647
Alexa Fluor 700 NBP2-76654AF700
Alexa Fluor 750 NBP2-76654AF750
Biotin NBP2-76654B
DyLight 350 NBP2-76654UV
DyLight 405 NBP2-76654V
DyLight 488 NBP2-76654G
DyLight 550 NBP2-76654R
DyLight 594 NBP2-76654DL594
DyLight 650 NBP2-76654C
DyLight 680 NBP2-76654FR
DyLight 755 NBP2-76654IR
FITC NBP2-76654F
HRP NBP2-76654H
Janelia Fluor 549 NBP2-76654JF549
Janelia Fluor 646 NBP2-76654JF646

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Bioinformatics

Gene Symbol ALPP